食品与发酵工业2026，Vol.52Issue(17)：31-38,8.DOI:10.13995/j.cnki.11-1802/ts.046495
基于常温室压等离子体诱变选育抗腹泻乳酸片球菌的性能评价及基因组重测序分析
Performance evaluation and genome resequencing analysis of anti-diarrhea Pediococcus acidilactici based on atmospheric and room temperature plasma mutagenesis
袁嘉怡 1付桂明 2范浩伟 3饶丽娟 1黄莉 1邱银涓 1万茵1
作者信息
- 1. 南昌大学食品科学与资源挖掘全国重点实验室,江西南昌,330047
- 2. 南昌大学食品科学与资源挖掘全国重点实验室,江西南昌,330047||江西南大国创院食品科技有限公司,江西南昌,330299
- 3. 江西南大国创院食品科技有限公司,江西南昌,330299
- 折叠
摘要
Abstract
Diarrhea remains a serious threat to human health,and the development of safe and effective microecological preparations is an important alternative to antibiotic therapy.A laboratory-preserved strain of Pediococcus acidilactici served as the parental strain for at-mospheric and room temperature plasma mutagenesis.A mutant with significantly enhanced antibacterial activity was screened and then evaluated for acid tolerance,bile salt tolerance,survival under simulated gastrointestinal conditions,auto-aggregation,cell surface hydro-phobicity,and safety.Genome resequencing was further conducted to identify key mutant genes associated with the improved antibacterial activity.The antibacterial activity of the mutant increased by 22.85％compared with that of the parental strain.The survival rates were 62.99％at pH 3.0 and 73.80％in 0.3％bile salts.Auto-aggregation and cell surface hydrophobicity reached 77.83％and 58.19％,re-spectively.The survival rates in simulated gastric fluid and simulated intestinal fluid were 60.58％and 55.57％,respectively.The mutant also showed a high level of safety.Genome resequencing identified three categories of differential genes related to the enhanced antibacteri-al activity.In conclusion,a Pediococcus acidilactici mutant with high antibacterial activity was successfully obtained by ARTP mutagene-sis.This study provides a promising strain resource and a theoretical basis for the development of novel microecological preparations for the prevention and treatment of diarrhea.关键词
乳酸片球菌/常温室压等离子体技术/抑菌性能/益生性能/基因组重测序Key words
Pediococcus acidilactici/atmospheric and room temperature plasma technology/antibacterial properties/probiotic perform-ance/genome resequencing引用本文复制引用
袁嘉怡,付桂明,范浩伟,饶丽娟,黄莉,邱银涓,万茵..基于常温室压等离子体诱变选育抗腹泻乳酸片球菌的性能评价及基因组重测序分析[J].食品与发酵工业,2026,52(17):31-38,8.
