食品与发酵工业2026，Vol.52Issue(17)：39-47,中插5-中插7,12.DOI:10.13995/j.cnki.11-1802/ts.045207
副干酪乳酪杆菌CD12-1的筛选、益生特性及基因组安全性评价
Screening,probiotic properties,and genomic safety assessment of Lacticaseibacillus paracasei CD12-1
摘要
Abstract
Traditional fermented dairy products are important sources of lactic acid bacteria(LAB).To screen LAB with probiotic po-tential from the Qinghai-Tibet Plateau,101 candidate strains were isolated from traditional fermented yak milk in Xinjiang and Tibet.Strain characteristics were systematically evaluated,including acid tolerance,bile salt tolerance,simulated gastrointestinal fluid tolerance,auto-aggregation,surface hydrophobicity,and antioxidant activity.Safety testing and whole-genome sequencing(WGS)were also conducted on the screened strains.The results showed that the strain CD12-1 exhibited the best overall performance,with acid and bile salt tolerance rates of 73.34％and 37.43％,respectively.Survival rates in simulated gastric and intestinal fluids were 121.63％and 76.57％.Auto-ag-gregation and surface hydrophobicity reached 91.19％and 82.97％,and DPPH radical scavenging activity was 72.17％.The strain was sensitive to antibiotics such as erythromycin,penicillin,and cefazolin,and showed no hemolytic activity.Based on 16S rRNA gene se-quence analysis,this strain was identified as Lacticaseibacillus paracasei.Whole-genome sequencing results revealed that its genome length was 2 483 754 bp with a GC content of 46.5％,containing 2 844 protein-coding genes.Functional annotation indicated that the strain's genome was rich in genes related to acid resistance,adhesion,transport,bacteriocins,and cold/heat shock proteins,and no risk genetic elements with horizontal transfer potential were detected.In conclusion,CD 12-1 shows strong probiotic potential and high safety,and its genomic features support its potential application use as a probiotic.关键词
传统发酵牦牛乳/乳酸菌/益生特性/全基因组测序Key words
traditional fermented yak milk/lactic acid bacteria/probiotic properties/whole genome sequencing引用本文复制引用
甄腾,李珺洋,李宏强,赵祥民,孔婷,唐德富..副干酪乳酪杆菌CD12-1的筛选、益生特性及基因组安全性评价[J].食品与发酵工业,2026,52(17):39-47,中插5-中插7,12.基金项目
甘肃省重点研发计划项目(22YF7NA113) （22YF7NA113）
甘肃省高校产业支撑计划项目(2023CYZC-47) （2023CYZC-47）
甘肃农业大学学科团队项目(GAU-XK-TD-2022-24) （GAU-XK-TD-2022-24）
甘肃省科技重大专项计划子课题项目(23ZDWA004) （23ZDWA004）
甘肃省高校研究生"创新之星"项目(2026CXZX-779) （2026CXZX-779）
