食品与发酵工业2026，Vol.52Issue(17)：48-54,7.DOI:10.13995/j.cnki.11-1802/ts.046739
无细胞系统催化己二酸合成1,6-己二胺研究
Synthesis of 1,6-hexanediamine from adipic acid catalyzed by a cell-free system
摘要
Abstract
1,6-Hexanediamine is an important monomer for polyamide materials such as nylon 66,and the development of a biosyn-thetic route that is independently controllable,mild,and sustainable is of great significance.In this study,a cell-free catalytic system composed of carboxylic acid reductase and transaminase was constructed to improve the efficiency of 1,6-hexanediamine production from adipic acid.The efficiency of cascade flux transfer was enhanced through screening of key enzyme combinations and process intensifica-tion.The results showed that the titer of 1,6-hexanediamine reached 36.5 mg/L in the purified enzyme catalytic system.After the introduc-tion of the cell-free catalytic system,the kinetic matching of the cascade reaction was improved and intermediate accumulation was allevia-ted,resulting in an increase in the titer of 1,6-hexanediamine to 329.7 mg/L.After further optimization of the reaction process,the maxi-mum titer of 1,6-hexanediamine reached 571.8 mg/L at 9 h.These results lay a foundation for the green biomanufacturing of 1,6-hexane-diamine and the process intensification of cell-free systems,and provide technical support for the further development of related biomanu-facturing processes.关键词
1,6-己二胺/生物法合成/无细胞催化/羧酸还原酶/转氨酶Key words
1,6-hexanediamine/biosynthesis/cell-free catalysis/carboxylic acid reductase/transaminase引用本文复制引用
刘韧,李丑强,毛银,李国辉,邓禹..无细胞系统催化己二酸合成1,6-己二胺研究[J].食品与发酵工业,2026,52(17):48-54,7.基金项目
国家自然科学基金项目(22578171) （22578171）
呼和浩特市科技突围工程揭榜挂帅项目(2025-科技突围-农社-3) （2025-科技突围-农社-3）
江苏省前沿技术研发项目(BF2025282) （BF2025282）
