中国食品添加剂2026，Vol.37Issue(9)：17-27,11.DOI:10.19804/j.issn1006-2513.2026.9.003
不同产地多花黄精体外抗氧化活性及其组分关联度分析
Correlation analysis between in vitro antioxidant activity and chemical components of Polygonatum cyrtonema from different geographic origins
摘要
Abstract
In order to establish ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS)chemical fingerprints of Polygonatum cyrtonema from different geographic origins,and to analyze their correlation with in vitro antioxidant activity to screen for potential antioxidant-related quality markers(Q-markers),ethanol extracts of 12 batches of P.cyrtonema collected from Sichuan and Guangxi provinces were prepared.UPLC-Q-TOF-MS was employed to generate chemical fingerprints and evaluate similarity among the batches.Antioxidant capacities were assessed using DPPH and ABTS free radical scavenging assays,and IC50 values were calculated.Grey relational analysis(GRA)and partial least squares regression(PLSR)were combined to determine the correlation between common peaks and antioxidant activity.Key peaks were subsequently identified based on high-resolution MS/MS data.The results showed that significant differences in DPPH and ABTS scavenging capacities were observed among samples from different origins,matching the variations found in fingerprint profiles.Correlation analysis revealed that peaks No.38,28,and 11 strongly correlated with DPPH scavenging activity,while peaks No.51,38,28,and 48 were primarily associated with ABTS scavenging activity.Integrated GRA-PLSR analysis identified peaks No.38,28,11,and 48 as the primary antioxidant-related peaks.Based on MS/MS data,these were tentatively assigned as oleoylethanolamide,p-hydroxy-N-(p-hydroxyphenethyl)cinnamamide,salicylic acid,and genistein,respectively.In conclusion,combining UPLC-Q-TOF-MS fingerprinting with GRA-PLSR correlation analysis effectively bridged the chemical differences in P.cyrtonema with its in vitro antioxidant efficacy.The identified compounds could serve as candidate quality markers,providing a valuable reference for the origin-based quality evaluation and standardization of P.cyrtonema.关键词
多花黄精/UPLC-Q-TOF-MS/指纹图谱/抗氧化活性/组分关联度分析/灰色关联分析/偏最小二乘回归Key words
Polygonatum cyrtonema/UPLC-Q-TOF-MS/fingerprint/antioxidant activity/component correlation analysis/grey relational analysis/partial least squares regression分类
轻工纺织引用本文复制引用
彭思蓉,李月,杨丽,张志锋..不同产地多花黄精体外抗氧化活性及其组分关联度分析[J].中国食品添加剂,2026,37(9):17-27,11.基金项目
四川省科技厅区域创新合作项目(2026YFHZ0137) （2026YFHZ0137）
西南民族大学青藏高原研究科技创新团队(2024CXTD22) （2024CXTD22）
中央高校基本科研业务费专项基金研究类项目(ZYN2026267) （ZYN2026267）
