中外葡萄与葡萄酒Issue(5)：7-17,11.DOI:10.13414/j.cnki.zwpp.2026.05.002
ABA调控的VvWRKY40对葡萄VvDXS表达及单萜合成的影响
Effects of ABA-Regulated VvWRKY40 on VvDXS Expression and Monoterpene Biosynthesis in Grapevine
摘要
Abstract
To elucidate how ABA-regulated VvWRKY40 affects VvDXS expression and monoterpene biosynthesis in grapevine,DNA affinity purification sequencing(DAP-seq)was used to screen candidate target genes,followed by expression correlation analysis,luciferase reporter assays,yeast one-hybrid assays,stable transformation of grapevine callus,transient transformation of grape berries,and ABA treatment.The two DAP-seq technical libraries yielded 21 853 non-redundant binding peaks,and the most significantly enriched motif contained a conserved TTGAC core sequence.Promoter localization,reproducibility filtering between the two libraries,and functional annotation review identified seven candidate genes related to volatile terpene biosynthesis,among which VvDXS showed a relatively strong negative correlation with VvWRKY40 expression.The 2,000-bp upstream promoter region of VvDXS contained three W-boxes,and the yeast one-hybrid assay demonstrated the binding of VvWRKY40 to the VvDXS promoter.Overexpression of VvWRKY40 in both grape berries and grapevine callus reduced VvDXS expression and the contents of linalool and geraniol,whereas ABA treatment downregulated VvWRKY40,upregulated VvDXS,and promoted monoterpene accumulation.Collectively,VvDXS may be a candidate target gene through which VvWRKY40 regulates monoterpene biosynthesis in grapevine,and ABA may affect this process by modulating the VvWRKY40-VvDXS module.关键词
葡萄/VvWRKY40/DAP-seq/VvDXS/脱落酸/单萜Key words
grapevine/VvWRKY40/DAP-seq/VvDXS/abscisic acid/monoterpene分类
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李相怡,罗阳,闫玥,开建荣,张静,陈翔,葛谦..ABA调控的VvWRKY40对葡萄VvDXS表达及单萜合成的影响[J].中外葡萄与葡萄酒,2026,(5):7-17,11.基金项目
宁夏回族自治区自然科学基金一般项目(2025AAC030304)重庆市自然科学基金面上项目(CSTB2023NSCQ-MSX0947)宁夏回族自治区重点研发计划项目(2024BBF02005) （2025AAC030304）
