食品科学2026，Vol.47Issue(19)：322-329,8.DOI:10.7506/spkx1002-6630-20260330-232
乳品中香兰素溯源与定量检测方法的建立及应用
Establishment and Application of Methods for Traceability and Quantitative Detection of Vanillin in Dairy Products
摘要
Abstract
An accurate analytical method based on ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry(UPLC-MS/MS)was established for the quantification of vanillin in dairy products.Additionally,a gas chromatography-combustion-isotope ratio mass spectrometry(GC-C-IRMS)method was developed for the traceability and identification of vanillin.The sample pretreatment conditions and the UPLC-MS/MS parameters were optimized,and the methods were validated systematically.The UPLC-MS/MS method showed a limit of detection(LOD)of 0.3 μg/kg and a limit of quantification(LOQ)of 1.0 μg/kg,with recoveries at different spiked levels ranging from 97.30%to 100.80%,demonstrating excellent sensitivity and accuracy.Matrix effect verification indicated that the pretreatment process did not induce significant isotope fractionation,confirming that the GC-C-IRMS method was stable and reliable.The established methods were applied to the analysis of 26 commercial dairy samples.The UPLC-MS/MS method enabled accurate quantification of trace vanillin,with positive results primarily found in formula milk((87.47±0.91)mg/kg).Traceability analysis by GC-C-IRMS confirmed that the vanillin detected in the positive samples with high vanillin content was synthetic.While UPLC-MS/MS provided high-sensitivity quantification,it could not distinguish the sources of the analyte.The complementarity of these two techniques highlights the significance of combining"precise quantification"with"stable isotope traceability"in differentiating between natural and synthetic vanillin in dairy products.This study achieves the deep integration of vanillin quantification and traceability,providing a rigorous technical tool for the scientific determination of vanillin sources.关键词
香兰素/乳品/超高效液相色谱-三重四极杆串联质谱/气相色谱-燃烧-同位素比值质谱/来源鉴别Key words
vanillin/dairy products/ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry/gas chromatography-combustion-isotope ratio mass spectrometry/source identification分类
轻工纺织引用本文复制引用
李文君,许秀丽,尹睿杰,韩晓旭,裴晓燕,廉洁,聂雪梅,沈正超,王秀娟..乳品中香兰素溯源与定量检测方法的建立及应用[J].食品科学,2026,47(19):322-329,8.基金项目
国家乳业技术创新中心项目(2024-KFKT-004) （2024-KFKT-004）
