食品与发酵工业2026，Vol.52Issue(18)：30-39,10.DOI:10.13995/j.cnki.11-1802/ts.045559
利用木糖合成β-榄香烯的解脂耶氏酵母构建及其发酵优化
Pathway engineering and medium optimization of Yarrowia lipolytica for β-elemene production from xylose
摘要
Abstract
β-Elemene is a sesquiterpene natural compound derived from Curcuma aromatica Salisb,a plant belonging to the Zingiber-aceae family.Endowed with excellent antitumor activity,it holds irreplaceable application value in the research and development of antitu-mor drugs as well as their clinical translation.To construct a high-efficiency microbial cell factory for β-elemene synthesis and establish a supporting high-yield fermentation process system,in this study,the engineered Yarrowia lipolytica strain LE-00 was used as the starting strain.A modular metabolic engineering strategy was adopted to perform stepwise rational regulation on the key functional genes involved in the β-elemene biosynthetic pathway,with a systematic analysis of the regulatory mechanism of gene expression patterns on the synthesis ef-ficiency of the target product.First,by strengthening the terpenoid precursor synthesis module,the engineered strain LE-08 was obtained,whose β-elemene yield reached(274.63±4.2)mg/L,representing a 38-fold increase compared with the control strain LE-01.On this basis,the copy number of the key fusion gene ERG20LLTC2 was further increased,which successfully elevated the yield of the target product to(413.28±49)mg/L.To further explore the production potential of the strain,single-factor experiments combined with the Box-Behnken response surface methodology were employed to systematically optimize the components of the fermentation medium.The opti-mal medium formulation was finally determined as follows,xylose 80 g/L,peptone 39.86 g/L,and yeast extract 17.93 g/L.Under these optimized conditions,shake-flask fermentation was carried out in baffled Erlenmeyer flasks,and the β-elemene yield could reach(912.33±70.93)mg/L.In conclusion,this study successfully established a high-efficiency biosynthetic technical route for β-elemene u-sing xylose as the carbon source,which provides important technical support and theoretical basis for reducing the industrial production cost of this compound,improving the economic benefits of the related biopharmaceutical industry,and promoting the industrialization process of natural antitumor drugs.关键词
β-榄香烯/解脂耶氏酵母/木糖代谢/发酵优化/合成生物学Key words
β-elemene/Yarrowia lipolytica/xylose metabolism/fermentation optimization/synthetic biology引用本文复制引用
乔燕,刘顺成,杨佳珂,邵语瑶,王柳欢,崔啸林,孙悦嘉,徐龙兴,袁丽杰,刘鹏才..利用木糖合成β-榄香烯的解脂耶氏酵母构建及其发酵优化[J].食品与发酵工业,2026,52(18):30-39,10.基金项目
国家自然科学基金项目(22501080) （22501080）
河北省自然科学基金项目(H2021209027,B2022209030) （H2021209027,B2022209030）
河北省教育厅青年人才拔尖计划(BJ2025069) （BJ2025069）
河北省中药管理局项目(2025434) （2025434）
2024年华北理工大学创新创业项目(329) （329）
2023年华北理工大学创新创业项目(T2023061) （T2023061）
