食品与发酵工业2026，Vol.52Issue(18)：40-47,8.DOI:10.13995/j.cnki.11-1802/ts.046510
金黄色类节杆菌来源的菊糖果糖转移酶在大肠杆菌中的高效表达及发酵条件优化
Efficient expression of inulin fructosyltransferase from Paenarthrobacter aurescens QL1.001 in Escherichia coli and optimization of fermentation conditions
摘要
Abstract
Difructose anhydride Ⅲ(DFA Ⅲ)is a novel functional sweetener characterized by enhanced mineral absorption,low ca-loric value,and excellent acid-base stability.Its bioproduction is critically dependent on inulin fructotransferase(IFTase).As the key en-zyme for industrial-scale synthesis of DFA Ⅲ,the development of high-yield IFTase-producing engineered strains and their corresponding optimized fermentation processes is of great importance.In this study,the IFTase gene derived from Paenarthrobacter aurescens QL1.001 was cloned and heterologously expressed in Escherichia coli,achieving an extracellular IFTase activity of 714.32 U/mL.Single-factor opti-mization was performed to improve the medium composition and fermentation conditions of the recombinant strain.The optimal medium(g/L)was determined as follows,tryptone 10.0,yeast extract 20.0,glycerol 5.0,K2HPO4 9.0,and KH2PO4 0.93.The optimal fer-mentation conditions were pH 8.0,24 ℃,0.6 mmol/L IPTG,and an induction time of 48 h.Under these optimized conditions,the IF-Tase activity increased to 1 449.83 U/mL,representing a significant increase compared with the initial shake-flask yield and approximate-ly a 20-fold improvement over the wild-type strain.This activity level was the highest in the literature for recombinant IFTase expression.These results demonstrated that the constructed recombinant E.coli strain and optimized fermentation conditions enabled the highly effi-cient production of IFTase,providing valuable technical parameters and theoretical support for the large-scale production of DFA Ⅲ.关键词
双果糖酐Ⅲ/菊糖果糖转移酶/异源表达/培养基优化Key words
difructose anhydride Ⅲ/inulin fructotransferase/heterologous expression/medium optimization引用本文复制引用
赵钰玉,崔佳欣,李腾超,赵萌,崔波..金黄色类节杆菌来源的菊糖果糖转移酶在大肠杆菌中的高效表达及发酵条件优化[J].食品与发酵工业,2026,52(18):40-47,8.基金项目
齐鲁工业大学(山东省科学院)科教产融合试点工程人才科研项目(2024RCKY017) （山东省科学院）
2024年度校(院)科教产融合试点工程重大创新专项(2024ZDZX05) （院）
山东省重点研发计划(重大科技创新工程)(2024CXGC010914) （重大科技创新工程）
