食品与发酵工业2026，Vol.52Issue(18)：48-54,7.DOI:10.13995/j.cnki.11-1802/ts.044774
基于组合代谢工程策略改造大肠杆菌合成L-色氨酸
Engineering Escherichia coli for L-tryptophan synthesis based on combined metabolic engineering strategies
周圣玉 1罗玮 1余晓斌1
作者信息
- 1. 江南大学生物工程学院,江苏无锡,214122
- 折叠
摘要
Abstract
L-tryptophan,an essential amino acid for humans,has wide applications in food,feed additives,and pharmaceuticals.This study applied systematic metabolic engineering to modify the L-tryptophan synthesis pathway,significantly improving its production.The tnaA gene was knocked out to reduce L-tryptophan degradation.Knockout of pheA,tyrA,and poxB genes eliminated carbon flux diver-sion through competing pathways.Global regulatory genes fruR,tyrR,and trpR were modified to redirect carbon flow.To enhance precursor supply,tktA and the galp-glk glucose transport system were integrated.Additionally,deleting the amn gene and overexpressing pntAB in-creased the supply of ATP and NADPH,optimizing energy metabolism.Furthermore,L-tryptophan secretion was enhanced while its uptake was reduced.These modifications resulted in the engineered strain XZ14.During fed-batch fermentation in a 5 L bioreactor,this strain a-chieved an L-tryptophan yield of 37.4 g/L,with a yield of 16.7％from glucose.These results establish a solid foundation for industrial application.关键词
L-色氨酸/代谢工程/合成生物学Key words
L-tryptophan/metabolic engineering/synthetic biology引用本文复制引用
周圣玉,罗玮,余晓斌..基于组合代谢工程策略改造大肠杆菌合成L-色氨酸[J].食品与发酵工业,2026,52(18):48-54,7.
