食品与发酵工业2026，Vol.52Issue(18)：314-321,8.DOI:10.13995/j.cnki.11-1802/ts.046624
甲壳素酶与甲壳素脱乙酰酶协同降解胶体甲壳素的效果研究
Study on effect of chitinase and chitin deacetylase on synergistic degradation of colloidal chitin
梁凤云 1蔡俊1
作者信息
- 1. 湖北工业大学生命科学与健康工程学院,湖北武汉,430000
- 折叠
摘要
Abstract
This study explored the synergistic degradation of colloidal chitin by chitinases(CSChiG,CSChiL,CSChiO)and chitin deacetylase AnCDA.The dual-enzyme system of endo-chitinase CSChiL and AnCDA showed the highest efficiency,with synergistic degrees of 2.08 and 1.5 at an enzyme activity ratio of 2∶1,and optimal reaction conditions of 50℃and pH 6.0.High performance liquid chroma-tography(HPLC),matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS),and Fourier transform infrared spectroscopy(FT-IR)analyses revealed that the system produced(GlcNAc)2 at(1.99±0.01)mg/mL(1.7-fold of single en-zyme)after 8 h,and reducing sugars 2.13-fold higher than CSChiL alone after 12 h.Synergy enhanced AnCDA's deacetylation activity,and after 24 h,the residual insoluble fraction had a deacetylation degree of(49.8±0.65)％and colloidal chitin's molecular weight de-creased to(64.4±0.9)kDa.These findings provide novel insights and practical parameters for chitin degradation.关键词
甲壳素酶/甲壳素脱乙酰酶/胶体甲壳素/协同降解Key words
chitinase/chitin deacetylase/colloidal chitin/synergistic degradation引用本文复制引用
梁凤云,蔡俊..甲壳素酶与甲壳素脱乙酰酶协同降解胶体甲壳素的效果研究[J].食品与发酵工业,2026,52(18):314-321,8.
