食品与发酵工业2026，Vol.52Issue(18)：330-337,8.DOI:10.13995/j.cnki.11-1802/ts.045143
基于金纳米棒-铜沉积协同信号放大的免疫层析试纸条检测玉米中黄曲霉毒素B1
Detection of aflatoxin B1 in maize using immunochromatographic test strip based on gold nanorod-copper deposition synergistic amplification
摘要
Abstract
Aflatoxin B1(AFB1)is a highly carcinogenic mycotoxin that poses a severe threat to human and animal health.In this study,an immunochromatographic test strip(ICTS)based on gold nanorod-copper deposition synergistic amplification was established for the highly sensitive detection of AFB,in maize.Specifically,copper deposition was employed to increase the particle size of gold nanorods(AuNRs),which in turn enhanced the color development intensity of the test strip and thereby improved the detection sensitivity.Under optimal conditions,the limit of detection(LOD)of the test strip after copper deposition was as low as 0.7 μg/kg,and its sensitivity was 4.9 times higher than that of the test strip without copper deposition.The spiked recovery rates ranged from 89.06％to 118.2％,with the coefficient of variation(CV)between 5.92％and 9.10％.No significant cross-reactivity with other mycotoxins was observed.This method provides an effective technical means for the rapid and sensitive detection of AFB1 in maize.关键词
黄曲霉毒素B1/金纳米棒/铜沉积/免疫层析试纸条Key words
aflatoxin B1/gold nanorods/copper deposition/immunochromatographic strip引用本文复制引用
曾峻涛,边瑞丽,任文洁,韩雨秀,李佳妮,任凯婵,何保山..基于金纳米棒-铜沉积协同信号放大的免疫层析试纸条检测玉米中黄曲霉毒素B1[J].食品与发酵工业,2026,52(18):330-337,8.基金项目
河南工业大学国际教育学院本科教育教学改革研究与实践项目(GJXY202405) （GJXY202405）
国家自然科学基金青年基金项目(32402234) （32402234）
河南省教育厅项目(25B550002) （25B550002）
