不同产地仙茅药材的HPLC指纹图谱研究OACSCDCSTPCD
Studies on HPLC Fingerprints of Curculigo orchioides from Different Regions
目的:建立仙茅药材的指纹图谱,并比较不同产地仙茅指纹图谱的差异,为科学评价仙茅的质量提供参考依据.方法:采用高效液相色谱法.色谱柱为Diama(钻石)C18(250 mm×4.6 mm,5 μm),流动相为甲醇-0.1%乙酸水溶液(梯度洗脱),检测波长为285 nm,柱温为30℃,流速为l mL·min 1,洗脱时间为80 min.结果:指纹图谱研究的各项参数均符合有关规定.仙茅不同产地17批药材中有14批的指纹图谱相似度较高,有13个共有峰,各…查看全部>>
OBJECTIVE: To establish the fingerprints of Curculigo orchioides, and to compare the difference of fingerprints of C. Orchioides so as to provide scientific basis for quality evaluation of C. Orchioides. METHODS: HPLC method was adopted. The separation was performed on Diama C18 column (250 mm×4.6 mm,5 μm)with mobile phase consisted of methanol-0.1 % acetic acid (gradient elution) at the flow rate of 1 mL· min-1. The UV detection wavelength was set at 285 nm…查看全部>>
马鹏;李隆云;喻强;卫莹芳;钟国跃
重庆市中药研究院,重庆市400065重庆市中药良种选育与评价工程技术研究中心,重庆市400065重庆市中药研究院,重庆市400065重庆市中药良种选育与评价工程技术研究中心,重庆市400065重庆市中药研究院,重庆市400065
医药卫生
产地仙茅高效液相色谱法指纹图谱
Regions Curculigo orchioides HPLC Fingerprints
《中国药房》 2011 (39)
3692-3694,3
国家“十五”科技攻关计划课题(2001BA701A32)
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