牛肌生成抑制素基因酵母双杂交诱饵载体的构建及鉴定OA北大核心CSCDCSTPCD
Construction of Myostain Gene Bait Vector in Bovine and Verification in Yeast Double-hybrid System
采用RT- PCR技术扩增牛肌生成抑制素基因成熟片段,并克隆入质粒pSos中,测序鉴定后获得酵母双杂交诱饵重组质粒pSos-MSTN.将诱饵重组质粒与对照质粒转化入酵母菌cdc25Hα中.结果表明:该段基因表达的蛋白对酵母菌cdc25Hα无毒性和无自激活作用,这说明可以用此酵母双杂交系统对牛肌生成抑制素进行研究.
The myostain gene was obtained by using RT-PCR and ligated with the pSos vector. After being verified by sequencing, the recombinant bait vector used in the yeast double-hybrid system was syn-thetized successfully. pSos-MSTN and control plasmids were cotransformed into the cdc25Ha yeast strain. The result shows that the pSos-MSTN vector was not toxic to cdc25Ha and had no self-activation. So this yeast double-hybrid system can be used to study myostain.
郑海舰;王军;柴孟龙;郑立双;窦长友;赵静
吉林农业大学动物科学技术学院,长春130118辽宁医学院畜牧兽医学院,锦州121001吉林农业大学动物科学技术学院,长春130118吉林农业大学动物科学技术学院,长春130118洮南市畜牧业管理局,洮南137100吉林农业大学动物科学技术学院,长春130118
农业科技
牛肌生成抑制素酵母双杂交系统诱饵载体
cattlemyostaindouble-hybrid systembait vector
《吉林农业大学学报》 2012 (2)
牛肌生成抑制素受体分子生物学特性研究
180-183,4
国家自然科学基金项目(30972108)
评论