吉林农业大学学报2012，Vol.34Issue(3)：339-342,354,5.
锦鲤疱疹病毒TaqMan荧光定量PCR快速检测方法的建立及应用
Establishment and Application of the TaqMan Real-Time PCR Detection Assay for Koi Herpesvirus
摘要
Abstract
To establish a rapid and effective quarantine method of the Kio Hepesvinis disease, a set of primers and TaqMan probe for Fluorescent Quantitation PCR were designed to detect the conservative sequence of Kio Hepesvirus Sph genes. The cell cultures were detected by using the established quantitative PCR assay, and the results were compared with those of the routine PCR. The quantitative PCR sensitivity was higher than that of the routine PCR. The quantity of KHV DNA was 1.6×102 copies/μL. A reliable diagnostic result can be obtained just within 4 h. The assay proved to be a rapid, sensitive, specific and repetitive method for rapid detection of Kio Hepesvirus from fish in quarantine.关键词
锦鲤疱疹病毒/荧光定量PCR/检测Key words
koi herpesvirus/flurogenic quantitative polymerase chain reaction/detection分类
农业科技引用本文复制引用
孟庆峰,吕文雪,单晓枫,陈东升,王伟利,钱爱东..锦鲤疱疹病毒TaqMan荧光定量PCR快速检测方法的建立及应用[J].吉林农业大学学报,2012,34(3):339-342,354,5.基金项目
吉林省科技发展计划项目(20080218) （20080218）
吉林农业大学学报
OA北大核心CSCDCSTPCD
1000-5684
访问量0
| 下载量0
- 1.锦鲤疱疹病毒实时荧光定量PCR检测方法的建立及应用
2022-06-23 04:07:55
- 2.TaqMan 荧光定量 PCR 快速检测锦鲤疱疹病毒方法的建立与应用
2022-06-23 15:19:49
- 3.锦鲤疱疹病毒潜伏感染实时荧光定量PCR检测方法的建立
2022-06-23 11:05:14
- 4.锦鲤疱疹病毒微滴式数字PCR检测方法的建立及临床应用
2025-03-05 02:00:32
- 5.猪嗜淋巴疱疹病毒3型TaqMan荧光定量PCR检测方法的建立及应用
2022-06-23 15:26:54