独行菜LaMCT基因的克隆、序列分析与原核表达OA北大核心CSCDCSTPCD
Cloning, sequence analysis and prokaryotic expression of LaMCT gene from Lepidium apetalum
强心苷作为药用植物独行菜( Lepidium apetalum)的活性成分,其化学和药理学研究已有良好的基础,但其生物合成途径目前仍不清楚。该研究以独行菜幼苗为材料,通过分析独行菜转录组数据,设计特异性引物,PCR扩增得到了强心苷生物合成MEP途径的关键酶2-C-甲基赤藓醇-4-磷酸胞苷酰转移酶( MCT)基因的开放阅读框( ORF),命名为LaMCT( Genbank注册号KT832554),并进行序列分析和原核表达。序列分析结果表明：LaMC…查看全部>>
The active components of Lepidium apetalum, cardiac glycosides have been well studied both chemically and pharmacologically, but little is known about the cardiac glycosides biosynthesis pathway. Using cultivated seedlings as mate-rial, the transcriptome data of Lepidium apetalum, designing specific primers and using PCR method, an open reading frame(ORF) of key enzyme 2-C-methylerythritol-4-phosphate cytidylyltransferase (MCT) gene which involved in MEP pat…查看全部>>
赵乐;马利刚;李晓阳;冯卫生;郑晓珂
河南中医学院 药学院，郑州450046 呼吸疾病诊疗与新药研发河南省协同创新中心，郑州450046河南中医学院 药学院，郑州450046 呼吸疾病诊疗与新药研发河南省协同创新中心，郑州450046河南中医学院 药学院，郑州450046
农业科技
独行菜MCT基因克隆序列分析原核表达
Lepidium apetalumMCTgene cloningsequence analysisprokaryotic expression
《广西植物》 2016 (10)
1225-1231,7
国家重点基础研究发展计划“973”项目(2013CB531802)；河南中医学院博士科研基金(BSJJ2011-07)[Supported by National Program on Key Basic Research Project (2013CB531802)；Doctoral Research Fund of Henan Chinese Medicine (BSJJ2011-07)]。
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