PCR技术鉴别多趾北京油鸡肉OA北大核心CSCDCSTPCD
Identification of Multi-Toed Beijing-You Chicken by PCR
北京油鸡是我国珍贵的地方品种,在自然群体中,部分个体表现出多趾性状,该性状是由于其LMBR1基因的246bp处发生突变,突变位点的碱基由C突变为A时表现出多趾形状.本实验根据多趾北京油鸡肉基因型与华都艾拔益加肉鸡基因型的不同,通过聚合酶链式反应(polymerase chain reaction,PCR)测序法和限制性酶切片段多态性(restriction fragment length polymorphism,RFLP)技术,确定突变位…查看全部>>
Beijing-you chicken is one of the precious local breeds in China. In the natural population, there are some multi-toed individuals because of the mutation of C-G base pair to an A-U pair at the 246 bp site in the LMBR1 gene.In this study, the genotype of multi-toed Beijing-you chicken was found to be different from that of Huadu Arbor Acres (AA) broiler, and the mutated base pair (A-U or C-G) was determined by PCR sequencing and restriction fragment polymorp…查看全部>>
陈静茹;夏海剑;张泽宾;曲鲁江;刘毅;李兴民
中国农业大学食品科学与营养工程学院,北京 100083中国农业大学动物科技学院,北京 100094中国农业大学动物科技学院,北京 100094中国农业大学动物科技学院,北京 100094中国农业大学食品科学与营养工程学院,北京 100083中国农业大学食品科学与营养工程学院,北京 100083
轻工纺织
北京油鸡艾拔益加肉鸡多趾PCR测序限制性酶切片段多态性(RFLP)
Beijing-you chickenHuadu Arbor Acres broilerpolydactlyPCR sequencingrestriction fragment polymorphism
《食品科学》 2017 (24)
229-234,6
北京市家禽产业创新团队项目(BAIC04-2017)
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