外源硒结合根瘤菌处理对蒙古黄芪生长及其活性成分的影响OACSTPCD

After treatment with exogenous selenium and rhizobium,the germination index of Astragalus membranaceus var.mongholicus(AMM)seeds was determined by germination bed test,and the growth index,antioxidant enzyme activity and active component content of AMM seedlings were determined by soil culture pot method.The results showed that exogenous selenium,rhizobium and exogenous selenium combined with rhizobium had significant inhibitory effects on the germination of AMM seeds(P＜0.05),but it promoted the growth of seedlings.Using exogenous selenium combined with rhizobium treatment,the SOD activity,POD activity and CAT activity of AMM seedlings increased 29.00%,27.90%and 46.31%,respectively,with significantly increase compared with the blank treatment(P＜0.05).The content of crude fat,nitrate nitrogen and total saponins in AMM were the highest with 4.77%,1.54%and 0.51%respectively in the combination of exogenous selenium and rhizobium treatment.Exogenous selenium combined with rhizobium significantly promoted antioxidant enzyme activity,crude fat,nitrate nitrogen and total saponin content of AMM seedlings.This study can lay a foundation and provide technical reference for the production and quality improvement of selenium-enriched Mongolian AMM for both medicine and food usage.