分析化学2024，Vol.52Issue(1)：13-21,9.DOI:10.19756/j.issn.0253-3820.231323
数字聚合酶链式反应技术及其在疾病检测中的研究进展
Development of Digital Polymerase Chain Reaction Technology and Its Progress in Disease Detection
摘要
Abstract
Digital polymerase chain reaction(dPCR)is a PCR technology that realizes accurate quantification of single-copy nucleic acid molecules by dividing the reaction system into tens of thousands of independent PCR reaction units for single-molecule-level amplification and integrating Poisson distribution.Due to its single-copy sensitivity and accurate quantification without the need of standard curves,dPCR has been widely used in disease diagnosis.By introducing technologies such as stepped emulsification and three-dimensional imaging,dPCR has been greatly improved in terms of accuracy,multiplexability and turnaround time,significantly enhancing its performance in clinical disease diagnosis.Based on this,this paper traced the technological development history of dPCR,gave an overview of its application in detection of tumors,infections and other diseases,and further discussed the challenges and opportunities of the development of dPCR,with the aim of providing a reference for the development and utilization of dPCR in the future,and promoting the high-quality development of molecular technology in clinical testing.关键词
数字聚合酶链式反应/微流控芯片/疾病诊断/单分子检测/评述Key words
Digtal polymerase chain reaction/Microfluidic chip/Disease diagnosis/Single molecule detection/Review引用本文复制引用
包旭东,胡孝林,万其武,张洪,罗阳..数字聚合酶链式反应技术及其在疾病检测中的研究进展[J].分析化学,2024,52(1):13-21,9.基金项目
国家自然科学基金项目(Nos.82125022,82241059,82202633)和中国博士后科学基金项目(No.BX20220194)资助. Supported by the National Natural Science Foundation of China(Nos.82125022,82241059,82202633)and the China Postdoctoral Science Foundation Funded Project(No.BX20220194). （Nos.82125022,82241059,82202633）
