层析法分离共轭亚油酸及异构体的实验研究OACSTPCD

Conjugated linoleic acid(CLA)is a generic term for the positional and conformational isomers of linoleic acid,which have been used in nutraceuticals.To further improve the safety of conjugated linoleic acid in food applications,substances with different roles in the conjugated linoleic acid were distinguished.Using column chromatography(also known as column chromatography),a novel column chromatography material was prepared using urea and silica gel for selectively adsorption of impurity components in conjugated linoleic acid products.Under the conditions of 1:9 diameter-to-height ratio and hexane as eluent,the content of conjugated linoleic acid could be increased by more than 6％in one column chromatography,and the content of two all-trans isomers of conjugated linoleic acid(including t 9,t11-CLA and t10,t12-CLA)decreased significantly;In the products subjected to the second column chromatography,the content of two all-trans structures of conjugated linoleic acid isomers decreased to about 0.2％,which effectively increased the safety of conjugated linoleic acid in applications.This study provides a new method for the purification of conjugated linoleic acid and reduces the potential risk of conjugated linoleic acid application in food.