超高效液相色谱-串联质谱法测定原花青素OACSTPCD

An ultra-performance liquid chromatography(UPLC)with mass spectrometry method was developed for determination of oligomeric proanthocyanidins(OPCs).Proanothocyanidins in the samples was reacted in acidified ethanol containing high iron ions under closed high temperature condition to generate cyanide ions,cyanide ions were separated by Zorbax Eclipse Plus C18 reverse phase chromatographic column(2.1 mm×50 mm,1.8 μm)with mobile phase(72:28,v/v)consisting of 1％formic acid and 28％methanol in water.Electrospray ionization(ESI)equipped with selected ion monitoring mode was utilized to determine a positive cyanide ion with a mass charge ratio of 287(m/z),and total proanothocyanidns content was quantitated by a USP certified OPCs reference standard.Excellent correlation coefficients of the linear curve of proanothocyanidins was achieved(R＞0.999)within the range of 0.2×10-6～2×10-6 g/mL,the average spike recovery at three different concentration levels was found to be within the range of 98％～104％,and RSD was less than 5％,demonstrated good precision and accuracy of the method.This study also found that different sources of reference standards resulted in different yielding rate of cyanidin,using oligomeric proanthocyanidin standards will help to ensure the consistency of proanthocyanidin determination results in the products.