蜡样芽孢杆菌GW-01全基因组测序及生物学特性分析OACSTPCD

Objective:To analyze the genome sequence information and biological properties of the pre-screened high-performance cypermethrin(β-CY)-degrading Bacillus cereus strain GW-01 through whole genome sequencing and bioinformatics,and to provide valuable insights for its safety assessment.Methods:The degradation ability of GW-01 towards β-CY was verified using HPLC.The whole genome of strain GW-01 was sequenced using third-generation single-molecule sequencing technology based on the second-generation Illumina NovaSeq and third-generation PacBio Sequel sequencing platforms.The sequencing data underwent analyses for genome assembly,gene prediction,functional annotation,prediction of carbohydrate-activating enzymes,virulence factors,and antibiotic resistance.Additionally,a phylogenetic tree was constructed for strain GW-01 based on the gyrA gene sequence.Results:On day 6,GW-01 degraded 48.4%of β-CY.The whole genome size of GW-01 strain was 5244145 bp with a cyclic genome type and an average GC content of 36.43%.It encoded a total of 5314 genes,including 104 tRNA genes and 313 ncRNAs.The whole genome sequences of GW-01 were annotated in the GO,KEGG,COG,NR,Swiss-Prot,CAZy,VFDB,and CARD databases,yielding 3536,4714,4434,5290,3854,135,263 and 96 annotated genes,respectively.GW-01 exhibited higher GC content and fewer virulence factors and resistance genes compared to the other four closely related strains.Conclusion:GW-01 displayed a distinct evolutionary pathway and a lower number of toxin-encoding genes compared to common Bacillus cereus strains,suggesting its higher safety profile.