超高效液相色谱串联质谱测定儿童血液样本中高三尖杉酯碱含量OACSTPCD
Determination of Harringtonine in Blood Samples of Children by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
目的:建立并验证超高效液相色谱串联质谱(UPLC-MS/MS)测定儿童血液样本中高三尖杉酯碱含量的方法.方法:以Waters Xevo TQD为检测设备,Waters BEH C18(100 mm ×2.1 mm,1.7 μm)为色谱柱,采用甲醇为有机相,含0.1％甲酸的超纯水为无机相,进行梯度洗脱,质谱检测采用正离子,多重反应监控(MRM)模式,待测物高三尖杉酯碱(HHT)离子通道m/z 546.3→298.2,内标物三尖杉酯碱(HT)离子通道…查看全部>>
Objective:To establish and verify ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for content determination of homoharringtonine in blood sample of children.Methods:Waters Xevo TQD was used as detection equipment,with Waters BEH C18(100 mm ×2.1 mm,1.7 μm)as separation column.Methanol as organic phase and ultra-pure water containing 0.1％formic acid as inorganic phase were used for gradient elution.Mass detection type wa…查看全部>>
袁树伟;杜小换;张先明;花盛浩;胡绍燕;朱增燕
苏州大学附属儿童医院,江苏苏州 215123苏州大学附属儿童医院,江苏苏州 215123苏州大学附属儿童医院,江苏苏州 215123苏州大学附属儿童医院,江苏苏州 215123苏州大学附属儿童医院,江苏苏州 215123苏州大学附属儿童医院,江苏苏州 215123
药学
超高效液相色谱串联质谱高三尖杉酯碱药代动力学
ultra-high performance liquid chromatography-tandem mass spectrometryhomoharringtoninepharmacokinetics
《儿科药学杂志》 2024 (3)
16-19,4
2023年度苏州市科技发展计划指导性项目,编号SKJD2023192.
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