超高效液相色谱串联质谱测定儿童血液样本中高三尖杉酯碱含量OACSTPCD

Objective:To establish and verify ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for content determination of homoharringtonine in blood sample of children.Methods:Waters Xevo TQD was used as detection equipment,with Waters BEH C18(100 mm ×2.1 mm,1.7 μm)as separation column.Methanol as organic phase and ultra-pure water containing 0.1％formic acid as inorganic phase were used for gradient elution.Mass detection type was positive mode,with multiple reaction monitoring(MRM).The detection ion pair of harringtonine(HHT)was 546.3→298.2,the detection ion pair of internal standard harringtonine(HT)was 532.2→298.3,the cone energy was 50 V,and the collision energy was 28 V.Results:This method was characterized by simple sample pretreatment,fast analysis speed,and high selectivity and sensitivity,with a linear interval of 0.5 to 120.0 ng,a linear equation of Y=0.047,011,3X+0.007,035,8(r=0.999,1),a lower limit of quantification of 0.5 ng,an intra-day precision of RSD＜8.16％,an inter-day precision of RSD＜6.38％,and an accuracy of 94.00％ to 103.95％.Conclusion:This method is selective and sensitive,and can be applied to the pharmacokinetic study of HHT in children and the monitoring of blood drug concentration and other related work.