儿科药学杂志2024，Vol.30Issue(3)：16-19,4.DOI:10.13407/j.cnki.jpp.1672-108X.2024.03.004
超高效液相色谱串联质谱测定儿童血液样本中高三尖杉酯碱含量
Determination of Harringtonine in Blood Samples of Children by Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry
摘要
Abstract
Objective:To establish and verify ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method for content determination of homoharringtonine in blood sample of children.Methods:Waters Xevo TQD was used as detection equipment,with Waters BEH C18(100 mm ×2.1 mm,1.7 μm)as separation column.Methanol as organic phase and ultra-pure water containing 0.1％formic acid as inorganic phase were used for gradient elution.Mass detection type was positive mode,with multiple reaction monitoring(MRM).The detection ion pair of harringtonine(HHT)was 546.3→298.2,the detection ion pair of internal standard harringtonine(HT)was 532.2→298.3,the cone energy was 50 V,and the collision energy was 28 V.Results:This method was characterized by simple sample pretreatment,fast analysis speed,and high selectivity and sensitivity,with a linear interval of 0.5 to 120.0 ng,a linear equation of Y=0.047,011,3X+0.007,035,8(r=0.999,1),a lower limit of quantification of 0.5 ng,an intra-day precision of RSD＜8.16％,an inter-day precision of RSD＜6.38％,and an accuracy of 94.00％ to 103.95％.Conclusion:This method is selective and sensitive,and can be applied to the pharmacokinetic study of HHT in children and the monitoring of blood drug concentration and other related work.关键词
超高效液相色谱串联质谱/高三尖杉酯碱/药代动力学Key words
ultra-high performance liquid chromatography-tandem mass spectrometry/homoharringtonine/pharmacokinetics分类
医药卫生引用本文复制引用
袁树伟,杜小换,张先明,花盛浩,胡绍燕,朱增燕..超高效液相色谱串联质谱测定儿童血液样本中高三尖杉酯碱含量[J].儿科药学杂志,2024,30(3):16-19,4.基金项目
2023年度苏州市科技发展计划指导性项目,编号SKJD2023192. （）
