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首页|期刊导航|饲料研究|正交试验法优化紫苏叶中总黄酮提取工艺及体外抗氧化活性研究

正交试验法优化紫苏叶中总黄酮提取工艺及体外抗氧化活性研究OACSTPCD

Optimization of extraction process and study of in vitro antioxidant activity of total flavonoids from Perilla frutescens leaves by orthogonal test

中文摘要英文摘要

试验以紫苏叶为研究对象,在单因素试验基础上,通过正交试验优化紫苏叶总黄酮的提取工艺,探讨总黄酮的抗氧化活性.以紫苏叶总黄酮得率为考察指标,对乙醇浓度、液料比、提取温度和提取时间进行优化.结果表明,4个因素对紫苏叶总黄酮得率影响的主次顺序为:提取温度>乙醇浓度>液料比>提取时间.紫苏叶总黄酮最优提取工艺参数为乙醇浓度50%、液料比20mL/g、提取温度70 ℃和提取时间45 min.在此条件下,紫苏叶总黄酮得率为4.05%.紫苏叶总黄酮对DPPH自由基与羟自由基具有较强的清除作用,其IC5.值分别为160 mg/L和196 mg/L.研究表明,紫苏叶总黄酮具有较强的抗氧化性能,是一种优质的天然抗氧化物质.

Using Perilla frutescens leaves as the research subject,based on single-factor experiments,the extraction process of total flavonoids from Perilla frutescens leaves was optimized through orthogonal experimental design to explore the antioxidant activity of total flavonoids.The yield of total flavonoids from Perilla frutescens leaves was taken as the evaluation index,and ethanol concentration,liquid-to-material ratio,extraction temperature,and extraction time were optimized.The results showed that the main and secondary influencing factors on the yield of total flavonoids from Perilla frutescens leaves were in the following order:Extraction temperature>ethanol concentration>liquid-to-material ratio>extraction time.The optimal extraction parameters for total flavonoids from Perilla frutescens leaves were found to be an ethanol.concentration of 50%,a liquid-to-material ratio of 20 mL/g,an extraction temperature of 70 ℃,and an extraction time of 45 minutes.Under these conditions,the yield of total flavonoids from Perilla frutescens leaves was 4.05%.Total flavonoids from Perilla frutescens leaves exhibited strong scavenging effects on DPPH free radicals and hydroxyl radicals,with IC50 values of 160 mg/L and 196 mg/L,respectively.The study indicates that total flavonoids from Perilla frutescens leaves possess strong antioxidant properties,making them a high-quality natural antioxidant.

尹丽;孟学林

广西工业职业技术学院,广西南宁 530001

畜牧业

紫苏叶总黄酮正交试验抗氧化活性超声辅助提取

Perilla frutescens leavestotal flavonoidsorthogonal testantioxidant activityultrasound-assisted extraction

《饲料研究》 2024 (004)

99-103 / 5

广西高校中青年教师基础能力提升项目(项目编号:2017KY1171);广西自然科学基金项目(项目编号:2022GXNSFAA294001)

10.13557/j.cnki.issn1002-2813.2024.04.018

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