人工栽培暴马桑黄多糖提取工艺优化及其抗氧化性研究OACSTPCD

Ultrasound-assisted hot water extraction was used to extract the polysaccharides of Sanghuangporus baumii.The effects of solid-liquid ratio,ultrasound time,and extraction time on the extraction rate were studied by single factor experiment and optimized by Box-Behnken response surface method.The total reducing power of FRAP,the scavenging capacity of hydroxyl,ABTS and DPPH radicals were determined to evaluate the antioxidant capacity of polysaccharides from Sanghuangporus baumii.The antioxidative effects of polysaccharides from Sanghuangporus baumii were examined by H2O2-induced PC12 cell model,where morphological observation and MTT assay were performed.The results of single factor test and response surface experiments showed that the highest extraction rate of 1.31%of polysaccharides was achieved at the extraction conditions of 1∶30 g/mL solid-liquid ratio,15 min ultrasound,and 60 min extraction time.The IC50 values for DPPH·,·OH and ABTS+ radicals were 0.403,2.870 and 0.142 mg/mL,respectively,which indicated that the polysaccharides from Sanghuangporus baumii had good antioxidant capacity,and cell experiments proved that the polysaccharide from Sanghuangporus baumii had a protective effect on the oxidative damage model established by H2O2,this indicated that the polysaccharides from Sanghuangporus baumii had strong antioxidant capacity and inhibitory effects on cellular oxidative damage.