食品科学2024，Vol.45Issue(8)：37-44,8.DOI:10.7506/spkx1002-6630-20230530-281
芦荟大黄素对α-葡萄糖苷酶的抑制机理及其与阿卡波糖的协同作用
Inhibitory Mechanism of Aloe Emodin on α-Glucosidase and Synergistic Effect with Acarbose
摘要
Abstract
In order to investigate the interaction between aloe emodin and α-glucosidase,enzyme kinetics,ultraviolet(UV)spectroscopy,infrared(IR)spectroscopy and fluorescence spectroscopy were employed to investigate the inhibitory mechanism of aloe emodin on α-glucosidase and the synergistic effect of aloe emodin in combination with acarbose was also investigated in this study.The results showed that aloe emodin had better inhibitory effect on α-glucosidase as both a non-competitive and anti-competitive inhibitor when compared with acarbose.The results of UV spectroscopy indicated that a new complex was formed through the interaction between aloe emodin and α-glucosidase.The characteristic vibrations of amide groups in the IR spectra indicated that the structural conformation of α-glucosidase changed with the addition of aloe emodin.The results of fluorescence quenching experiments showed that the endogenous fluorescence of α-glucosidase was statically quenched by aloe emodin.In addition,it was also found that aloe emodin combined with acarbose had a synergistic inhibitory effect on α-glucosidase activity.This study provides an experimental basis for the future application of aloe emodin in health foods for regulating blood glucose.关键词
芦荟大黄素/α-葡萄糖苷酶/抑制机理/协同抑制/光谱分析Key words
aloe emodin/α-glucosidase/inhibitory mechanism/synergistic inhibition/spectral analysis分类
中医学引用本文复制引用
秦朝凤,李姣,郝经文,卜雅琴,李胜,陈乃东..芦荟大黄素对α-葡萄糖苷酶的抑制机理及其与阿卡波糖的协同作用[J].食品科学,2024,45(8):37-44,8.基金项目
安徽省中药质量评价与品质提升科研创新团队(2022AH010090) （2022AH010090）
安徽省级高校自然科学研究重点项目(2022AH051676) （2022AH051676）
安徽省中药资源保护与持续利用工程实验室重点科技攻关项目(TCMRPSU-2022-06) （TCMRPSU-2022-06）
