Effect of biochar on the metabolome of soybean seedlingsOA
Effect of biochar on the metabolome of soybean seedlings
The use of biochar can have several effects on plant germination,depending on raw material,preparation method and application dose.However,the molecular mechanisms that lead to those results have yet to be elucidated.The aim of this research was to improve the understanding of these mechanisms by characterizing the metabolic effects of sugarcane bagasse biochar on soybean germination.Three types of biochars were prepared by pyrolysis at 300 ℃(SCB300),400 ℃(SCB400)and 600 ℃(SCB600).Then,each one was mixed into sand at 1％,3％,5％(w/w)dose,respectively.The experiment was performed in 8 days of incubation,when the number of germinated seeds and the average radicle length were determined.To evaluate the metabolome,the dry biomass(DB)was subjected to extraction with a mixture of methanol-d4 and D2O(1∶1 v/v).The extracts were submitted to metabolomics analysis by Proton Nuclear Magnetic Resonance.The Relative Germination,Relative Average Radicle Growth and Germination Index increased in all treatments compared to control.On the other hand,the DB increased in all treatments,except for SCB300,at doses of 1％and 3％w/w.Seven metabolites(alanine,asparagine,acetic acid,citric acid,glycerol,fatty acids and sucrose)were identified and quantified in DB extracts as the most influential finding for the separation of treatments.Taken together,these results strongly suggested that biochars accelerated the catabolism of triacylglycerols to sucrose and induced a slight osmotic stress.
Nathalia E.Silva;Mariana G.Aguilar;Osania E.Ferreira;Gleicia M.Paulino;Jaqueline C.L.Carvalho;Lucia P.S.Pimenta;Alan R.T.Machado
Programa de Pós-graduação em Ciências Ambientais,Universidade do Estado de Minas Gerais,Frutal,Minas Gerais,38202-436,BrazilDepartamento de Química,Universidade Federal de Minas Gerais,Minas Gerais,Belo Horizonte,31270-901,BrazilDepartamento de Engenharia Aplicada e Tecnologias Ambientais,Universidade do Estado de Minas Gerais,João Monlevade,Minas Gerais,35930-314,BrazilDepartamento de Ciências Exatas,Universidade do Estado de Minas Gerais,João Monlevade,Minas Gerais,35930-314,BrazilPrograma de Pós-graduação em Ciências Ambientais,Universidade do Estado de Minas Gerais,Frutal,Minas Gerais,38202-436,Brazil||Departamento de Ciências Exatas,Universidade do Estado de Minas Gerais,João Monlevade,Minas Gerais,35930-314,Brazil
GerminationMetabolomics1HNMRPyrolysisSugarcane bagasse
《中国油料作物学报（英文）》 2024 (001)
60-68 / 9
The authors thank Fundação Coordenação de Aperfeiçoamento de Pessoal de Nível Superior(CAPES),Fundação de Amparo à Pesquisa do Estado de Minas Gerais(FAPEMIG[grant number APQ-02349-21])and Universidade do Estado de Minas Gerais(UEMG[Productivity Researcher of the UEMG-PQ/UEMG])for their financial support and fellowships.The authors acknowledge the BioAnalytical Facility NEPS-DQ(https://ne.qui.ufmg.br)at the Universidade Federal de Minas Ger-ais,Belo Horizonte,MG,for the support with NMR analyses of this work.
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