食品工业科技2024，Vol.45Issue(10)：376-385,10.DOI:10.13386/j.issn1002-0306.2023060216
微生物合成2'-岩藻糖基乳糖和3-岩藻糖基乳糖的代谢工程策略研究进展
Research Progress in Metabolic Engineering Strategies for Microbial Synthesis of 2'-Fucosyllactose and 3-Fucosyllactose
摘要
Abstract
Human milk oligosaccharides(HMOs)are key components of breast milk,playing a vital role in fostering the healthy development of infants and young children.Among the main HMOs components,2'-fucosyllactose(2'-FL)and 3-fucosyllactose(3-FL)exhibit considerable benefits in terms of inhibiting pathogenic infections,regulating gut microbiota,and enhancing immunity.In recent years,the synthesis of 2'-FL and 3-FL has emerged as a prominent area of research in the field.Despite the potential advantages of chemical or enzymatic synthesis of 2'-FL and 3-FL,these methods are hindered by their inherent limitations,including intricate reaction procedures,high costs,and poor yield,which are not conducive to meeting the demand of the market.Therefore,more efficient microbial methods have been developed and applied in the commercial production of 2'-FL and 3-FL.This article systematically outlines the methods for preparing both 2'-FL and 3-FL,thoroughly expounds upon the engineering strategies for microbial synthesis of 2'-FL and 3-FL,and provides a compelling framework for further microbial production exploration of 2'-FL and 3-FL.关键词
2'-岩藻糖基乳糖/3-岩藻糖基乳糖/微生物法/工程化策略Key words
2'-fucosyllactose/3-fucosyllactose/microbiological method/engineering strategy分类
生物科学引用本文复制引用
李邓斌,魏超,张媛,张根林,王小艳..微生物合成2'-岩藻糖基乳糖和3-岩藻糖基乳糖的代谢工程策略研究进展[J].食品工业科技,2024,45(10):376-385,10.基金项目
国家自然科学基金(22178226) （22178226）
国家重点研发计划"合成生物学"重点专项,合成生物学自动化铸造平台关键技术研发(2018YFA0902900) （2018YFA0902900）
"十四五"国家重点研发计划重点专项(2021YFD2101000,2021YFD2101002). （2021YFD2101000,2021YFD2101002）
