HPLC-MS/MS测定花椒及其制品中羟基-α-山椒素OACSTPCD

An analytical method for the determination of hydroxy-α-capsaicin in Zanthoxylum and its products by liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)was established.The samples were ultrasonically extracted twice by 70%methanol aqueous solution for 10 min each time,and eluted with 0.1%formic acid aqueous solution and methanol as mobile phase gradient,and were separated by C18 column,scanned in positive ion mode,and detected in multiplex reaction monitoring mode.Retention time and characteristic ion pair(precursor and two fragment ions)information comparison was qualitative,quantified by external standard.The results showed that in the range of 2～500 ng/mL,the correlation coefficient of the linear regression equation by hydroxy-α-capsaicin was 0.999,and the average recovery rate of the sample was 80.8%～94.4%,and the relative standard deviation(RSD)was 0.8%～4.9%.When the sample was weighed to 1 g and the volume was 25 mL,the limit of detection(LOD)was 0.05 mg/kg and the limit of quantification(LOQ)was 0.1 mg/kg.This method was efficient,fast,accurate and precise to meet the detection requirements,and could accurately determine the content of hydroxy-α-capsaicin.This paper can provide a reference for the quality control of zanthoxylum and its products.