现代食品科技2024，Vol.40Issue(4)：64-72,9.DOI:10.13982/j.mfst.1673-9078.2024.4.0521
大曲耐热细菌富集及其在小曲白酒中的应用
Enrichment of Thermotolerant Bacteria in Daqu and Its Application in Xiaoqu Liquor
摘要
Abstract
The thermotolerant bacterial flora in Daqu contains various enzyme systems,which considerably influence the production of flavor substances in liquor.The high-temperature enrichment method was used to isolate the target thermotolerant bacterial flora from Daqu,which was subsequently incorporated into bran koji and utilized in the brewing process of Xiaoqu liquor.The functions of the Daqu thermotolerant bacterial flora in Xiaoqu liquor under three high-temperature conditions were preliminarily explored.Microbial structure and diversity were analyzed using 16S rDNA sequencing,and the effect of moisture content on bacterial biomass in bran koji was investigated.The results showed that the thermotolerant bacterial flora,dominated by Bacillus,present in Daqu was successfully transferred in bran koji and became dominant.The increase in enrichment temperature within a certain range also led to an increase in the proportion of thermotolerant bacteria.When the moisture content was 55%,the bacterial biomass in bran koji was up to 8.52×1010 CFU/g.Brewing experiments using Xiaoqu liquor revealed that the optimal Xiaoqu liquor produced by brewing led to an increase in the liquor yield by 3.7%,a decrease in the content of higher alcohol by 27.27%,and an increase in the ester content by 23.07%,compared with the control group.关键词
大曲/耐热细菌/群落分析/麸曲/小曲白酒Key words
Daqu/thermotolerant bacteria/community analysis/bran koji/Xiaoqu liquor引用本文复制引用
应超,任志强,曾波,黄治国,张运祺,卫春会..大曲耐热细菌富集及其在小曲白酒中的应用[J].现代食品科技,2024,40(4):64-72,9.基金项目
四川轻化工大学泸州老窖研究生创新基金(LJCX2022-3) （LJCX2022-3）
四川省科技创新创业苗子工程项目(23MZGC0042) （23MZGC0042）
酿酒生物技术及应用四川省重点实验室开放基金项目(NJ2022-07) （NJ2022-07）
