不同温度对羊肚菌淀粉代谢的调控研究OACSTPCD

The effects of temperature on mycelial growth,amylase activity(adenosine diphosphate glucose-pyrophosphorylase AGPase,α-amylase,β-amylase,starch branching enzyme DBE,starch debranching enzyme SBE),starch content(amylopectin,amylopectin)and amylase-related gene expression of Morchella in starch medium were studied.The results showed that in the temperature range of 10-25 ℃,with the increase of temperature,the growth rate of mycelia on starch medium was accelerated,and the mycelial density and mycelial tip bifurcation were also increased.The activity of AGPase,DBE,α-amylase and β-amylase was the highest at 20 ℃,while the activity of SBE was the highest at 10 ℃.Both amylose content and amylopectin residual content were the highest at 10 ℃.In addition,correlation analysis results showed that temperature had no significant correlation with α-amylase activity and SBE activity,but had significant negative correlation with β-amylase activity,AGPase activity and DBE activity,had extremely significant negative correlation with amylose content and amylopectin content.According to the above results,morels had the highest metabolic capacity for starch in the culture medium at 15-20 ℃,and the temperature of 10 ℃ and 25 ℃ were not unfavorable for morels to use starch in the culture substrate.This study provided theoretical and data support for the artificial cultivation of Morchella.