上海农业学报2024，Vol.40Issue(2)：31-36,6.DOI:10.15955/j.issn1000-3924.2024.02.06
不同温度对羊肚菌淀粉代谢的调控研究
Regulation of starch metabolism in Morchella at different temperature
摘要
Abstract
The effects of temperature on mycelial growth,amylase activity(adenosine diphosphate glucose-pyrophosphorylase AGPase,α-amylase,β-amylase,starch branching enzyme DBE,starch debranching enzyme SBE),starch content(amylopectin,amylopectin)and amylase-related gene expression of Morchella in starch medium were studied.The results showed that in the temperature range of 10-25 ℃,with the increase of temperature,the growth rate of mycelia on starch medium was accelerated,and the mycelial density and mycelial tip bifurcation were also increased.The activity of AGPase,DBE,α-amylase and β-amylase was the highest at 20 ℃,while the activity of SBE was the highest at 10 ℃.Both amylose content and amylopectin residual content were the highest at 10 ℃.In addition,correlation analysis results showed that temperature had no significant correlation with α-amylase activity and SBE activity,but had significant negative correlation with β-amylase activity,AGPase activity and DBE activity,had extremely significant negative correlation with amylose content and amylopectin content.According to the above results,morels had the highest metabolic capacity for starch in the culture medium at 15-20 ℃,and the temperature of 10 ℃ and 25 ℃ were not unfavorable for morels to use starch in the culture substrate.This study provided theoretical and data support for the artificial cultivation of Morchella.关键词
羊肚菌/淀粉酶活/淀粉含量/基因表达Key words
Morchella/Amylase activity/Starch content/Gene expression分类
农业科技引用本文复制引用
白静,李欣苹,赵怡然,陈辉,张津京..不同温度对羊肚菌淀粉代谢的调控研究[J].上海农业学报,2024,40(2):31-36,6.基金项目
上海市科技兴农项目(2021-02-08-00-12-F00786) （2021-02-08-00-12-F00786）
