食品接触材料的抑菌性能测试中菌落显色方法的研制与应用OACSTPCD

A new method of bacterial testing in food-contact material was developed to overcome the problems of complex operation steps and long inspection cycles in traditional test methods.Cotton fibers and gelatin-based collection paper for sample collecting were prepared.The colorimetric media was prepared by 2,3,5-triphenyltetrazolium chloride and plate count agar.Home-made collection test strips and colorimetric culture media were introduced into the antibacterial performance testing of food contact materials,and a colony colorimetric method was constructed to compare and analyze the differences in colony counting results between the new colony colorimetric method and traditional methods.The results showed no statistically significant difference between the new colony colorimetric method and the traditional method for testing the antibacterial performance of food contact materials,and both methods had same representation and accuracy.However,the operation steps of the new colony colorimetric method were simple,with a short testing cycle due to no bacterial washing procedures and could be operated in batches.Therefore,the colony colorimetric method can replace the traditional method in various testing laboratories for supervision and evaluation of antibacterial performance of food-contact materials.