库尔勒香梨中性多糖结构及其抗氧化抗过敏性哮喘活性研究OACSTPCD

The structure and antioxidant activity of neutral polysaccharide(PSNP-1,PSNP-2)from Pyrus sinkiangensis Yu were detected,and effect and effect mechanism of neutral polysaccharide on mice with allergic asthma were investigated.Two components PSNP-1 and PSNP-2 were obtained from Pyrus sinkiangensis Yu by column chromatography.The total sugar content of PSNP-1 and PSNP-2 were 91.81%and 98.62%,respectively.Their uronic acid content was 2.94%and 3.11%,and their molecular weight was 1503.14 and 26.73 kDa,respectively.The monosaccharide composition of PSNP-1 were glucose,galactose,and arabinose with a ratio of 54.6∶5.1∶2.9,and a small amount of mannose and rhamnose.PSNP-2 was mainly composed of mannose,glucose,galactose and arabinose,with a ratio of 1.8∶38∶10.5∶33,and small amount of rhamnose.Both polysaccharides had absorption peaks of polyhydroxy sugars and neutral pyranose rings were connected by α-glucoside and β-glucoside bonds.In vitro antioxidant experiments showed that PSNP-1 and PSNP-2 had good antioxidant activities and could improv symptoms of airway inflammation of OVA-induced asthma in mice.The mechanism may be through the intervention of TLR4/NF-κB signaling pathway to improve the airway inflammation of OVA-induced asthma.