响应面法优化亚临界水提取艾草总黄酮的工艺研究OACSTPCD

A subcritical water extraction process was optimized by response surface experiments to extract total flavonoids from mugwort.Based on tests of single factors including extraction temperature,extraction time,liquid-solid ratio,and magnetic stirring speed,Design Expert13.0 software was used to design the extraction process optimization experiment.Response surface interaction analysis was used to establish multiple quadratic regression equation for subcritical water extraction of total flavonoids from mugwort.The optimal extraction conditions were obtained by regression analysis.The optimal extraction process of total flavonoids from mugwort was as follows:extraction time 20.8 min,extraction temperature 127.3℃,liquid-solid ratio 87.7:1 mL/g.The predicted value of 15.28%of mugwort total flavonoid extraction rate was very close with the experiment data,which indicated the model had certain feasibility.The structure of total flavonoids in mugwort was characterized by FT-IR and LC-MS,the results showed that the mugwort total flavonoids might contain daidzein.The extraction process was simple and reliable,economical,and environmentally friendly.The study provides a reference for flavonoids extraction.