山莴苣素和山莴苣苦素改善高脂饮食诱导的肥胖小鼠脂代谢及氧化应激水平OACSTPCD

Randomly divide HFD induced obese mice into normal diet group,high-fat diet group,colchicine group,colchicine group,orlistat group,and metformin group.After 10 weeks of feeding,various relevant indicators of each group of obese mice were detected using serum biochemical indicators detection,HE staining method,real-time fluorescence quantitative PCR method,in order to investigate the effects of Lactucin and Lactopicrin on improving lipid metabolism and oxidative stress in HFD-induced obese mice.The results showed that,Lactucin and Lactucopicrin significantly alleviated obesity caused by HFD in mice,reduced hepatic TG levels(P＜0.01)and hepatic fat vacuoles,and improved hepatic cord lesions.Lactucin and Lactucopicrin significantly elevated hepatic T-AOC and SOD levels,and significantly lowered MDA levels,Lactucopicrin significantly increased CAT levels(P＜0.05);Lactucin and Lactucopicrin significantly increased renal SOD levels and significantly decreased MDA levels,and Lactucin significantly increased T-AOC levels(P＜0.05).Both Lactucin and Lactucopicrin significantly down-regulated mRNA expression of lipid synthesis genes Srebp-1c and Fas(P＜0.05),and Lactucopicrin significantly up-regulated mRNA expression of lipid catabolic genes Pparα and Cpt-1α(P＜0.05).Lactucin significantly enhanced mRNA expression of hepatic Nrf2(P＜0.01),and both Lactucin and Lactucopicrin significantly enhanced mRNA expression of renal Nrf2(P＜0.001).The conclusion was Lactucin and Lactucopicrin could affect expression of lipid metabolism genes and antioxidant genes and improve lipid metabolism and oxidative stress levels in high-fat diet-induced obese mice.