异硫氰酸苄酯对荧光假单胞菌的抑菌活性及在牛肉保鲜中的应用OA北大核心CSTPCD

Pseudomonas fluorescens is widely found in raw animal products such as beef,fish,poultry and dairy products,and has become an important cause of foodborne diseases.The aim of this paper was to investigate the inhibitory effect of benzyl isothiocyanate(BITC)on P.fluorescens ATCC 13525 as a test strain and its application in beef for freshness preservation.The minimum inhibitory concentration(MIC)of BITC on P.fluorescens was observed to be 0.25 mmol/L by the Oxford cup method.Phenotyping experiments demonstrated that BITC was able to disrupt the permeability of bacterial cell membranes and reduce metabolic activity.In addition,compared with the control group,the total viable count,color difference,pH,total volatile basic nitrogen(TVB-N)content and nitrogen oxides content in the MIC-treated group were reduced by 35.73%,21.52%,4.53%,54.39%,and 4.78%,respectively,after 7 days of storage,and the sensory score increased by 10.45%,indicating that BITC could significantly inhibit the growth of P.fluorescens and improve the sensory quality and physicochemical indexes of beef.Therefore,BITC has a good application prospect in the preservation of chilled beef.