食品与发酵工业2024，Vol.50Issue(12)：336-342,7.DOI:10.13995/j.cnki.11-1802/ts.036734
基于非天然氨基酸的蛋白质合成研究进展
Research progress on protein synthesis based on unnatural amino acids
摘要
Abstract
Protein is an important component of living organisms and participates in almost all life activities.Under normal circum-stances,the protein in the organism is composed of 20 natural amino acids.However,with the rapid development of modern science and technology,the demand for medical treatment,food,materials,and other aspects of people's life is increasing,and these proteins can no longer meet the diversified application needs.At the same time,with the excellent performance of unnatural amino acids and their synthe-sized proteins are gradually becoming a research hot spot.The technology of modifying proteins with unnatural amino acids has been gradu-ally developed.Unnatural amino acids have different side chain groups from natural amino acids,which can bring new properties and func-tions to proteins.Based on the study of unnatural amino acids and unnatural proteins,this review first introduces the chemical and biologi-cal methods for synthesizing unnatural amino acids,and summarizes the advantages and disadvantages of chemical and biological methods and their future development prospects.Then several popular methods of incorporating unnatural amino acids into proteins are presented.Then,the applications of unnatural amino acids and their synthetic proteins in enzyme modification,scientific research and medical treat-ment are listed and described.Finally,the future development of this technology is prospected and summarized.关键词
非天然氨基酸/非天然蛋白质/细胞代谢工程/遗传密码扩展/酶的改造/蛋白药物改造Key words
unnatural amino acids/unnatural proteins/cell metabolic engineering/genetic code expansion/enzyme modification/pro-tein drug modification引用本文复制引用
赵梅,刘馨远,陈洋,商洁,丛宏日,马豪,RAVIKUMAR Yuvaraj,齐向辉..基于非天然氨基酸的蛋白质合成研究进展[J].食品与发酵工业,2024,50(12):336-342,7.基金项目
国家自然科学基金项目(32200061,32150410349) （32200061,32150410349）
江苏省自然科学基金项目(BK20210752) （BK20210752）
中国博士后项目(2023T160278,2022M721390) （2023T160278,2022M721390）
科技部国家外专局项目(WGXZ2022038L) （WGXZ2022038L）
