大豆浓缩磷脂脱杂规律及影响其透明度物质的研究OA北大核心CSTPCD

Objective:This study aimed to explore the impurity removal law of soybean lecithin concentrate,analyze and identify the substances that affect the transparency of soybean lecithin concentrate.Methods:Using food-grade soybean lecithin concentrate as raw material and light transmittance as the main evaluation index,the effects of ceramic membrane pore size,material-liquid ratio,solvent system(n-hexane,n-hexane-anhydrous ethanol)and solvent ratio on impurity removal of soybean lecithin concentrate were investigated.Moreover,the collected impurities and soybean concentrated lecithin before and after impurity removal were analyzed and identified by lipidomics.Results:In the system of n-hexane-anhydrous ethanol,the inorganic ceramic membrane with a pore size of 20 nm was selected,and the ratio of solid to liquid of food-grade soybean concentrated lecithin:n-hexane-anhydrous ethanol was 1:3 and the ratio of n-hexane to anhydrous ethanol was 3∶1,which could effectively remove the particulate and fat-soluble impurities that affected its transparency.Moreover,the lipid-soluble substances affecting the transparency of soybean condensed lecithin were identified as sugar ester monogalactose diacylglycerol and derivative ester dimethyl phosphatidylethanolamine by lipid proteomics analysis.Conclusion:The nano-scale ceramic membrane can effectively remove impurities from concentrated soybean lecithin in the system of n-hexane and absolute ethanol,and the transparency of the prepared products is better than that of imported transparent soybean lecithin.