中国食品添加剂2024，Vol.35Issue(6)：1-7,7.DOI:10.19804/j.issn1006-2513.2024.6.001
副球菌TH21-44胞外多糖的结构表征及其抗氧化活性探究
Structural characterization of exopolysaccharide from Paracoccus sp.TH21-44 and its antioxidant activity
摘要
Abstract
The exopolysaccharides(EPS)produced by Paracoccus sp.TH21-44 were isolated and purified by ion-exchange chromatography,and their chemical components and structure were analyzed by full-wave scanning,electron microscopy,high-performance liquid chromatography and gel permeation chromatography.The antioxidant activities of TH21-44 EPS was also evaluated in terms of DPPH,ABTS+,hydroxyl radical scavenging,and reduction abilities to assess its antioxidant potential.The results showed that the EPS of Paracoccus sp.TH21-44 consisted of two polysaccharide fractions,of which EPS-1 was the main component with 92.63%of total sugars,along with small amounts of protein and sulfate.The rough and dense porous structure of EPS-1 appeared flaky or branched.The monosaccharide composition of EPS-1 was dominated by glucose,galactose and mannose.The antioxidant results showed that TH21-44 EPS removed 59.61%of DPPH and 78.55%of hydroxyl radicals at a concentration of 5 mg/mL,and EPS-1 showed ABTS scavenging ability of 99.36%at a concentration of 3 mg/mL,equivalent to that of Vc.These findings provides valuable insights for the utilization of microbial exopolysaccharides as bioresources.关键词
副球菌/胞外多糖/结构表征/抗氧化Key words
Paracoccus sp./exopolysaccharide/structural characterizations/antioxidant分类
轻工纺织引用本文复制引用
马博文,张博源,黄亮,周佳,赵帅,黄峰,屈建航..副球菌TH21-44胞外多糖的结构表征及其抗氧化活性探究[J].中国食品添加剂,2024,35(6):1-7,7.基金项目
国家自然科学青年基金(42107139) （42107139）
郑州市R&D专项经费补助科研项目(22ZZRDZX32) （22ZZRDZX32）
河南工业大学创新基金支持计划专项(2021ZKCJ15) （2021ZKCJ15）
河南工业大学高层次人才科研启动基金项目(2022BS065) （2022BS065）
