中国食品添加剂2024，Vol.35Issue(6)：8-15,8.DOI:10.19804/j.issn1006-2513.2024.6.002
低共熔溶剂提取晋产吸水石栽培铁皮石斛多糖工艺优化及抗氧化活性研究
Optimization of extraction process of polysaccharides from Dendrobium officinale cultivated on water-absorbing stone in Shanxi Province using low eutectic solvents and its antioxidant activity
摘要
Abstract
Dendrobium officinale is a traditional plant in China used for both medicinal and edible purposes,with high traditional Chinese medicinal value,especially due to its significant polysaccharide content.Dendrobium officinale cultivated on water-absorbing stones in Shanxi province was taken as the raw materials,the polysaccharide extraction process was optimized using eutectic solvent extraction technique through single-factor and response surface analysis.Subsequently,the scavenging effects of different concentrations of polysaccharides on DPPH,hydroxyl radicals,and ABTS+were analyzed.The results showed that the optimized polysaccharide extraction process of Dendrobium officinale using a L-proline-oxalic acid eutectic solvent system was as follows:with a material-to-liquid ratio of 1∶21(g/mL),the temperature of 56℃,under ultrasound for 30 minutes,resulted in a polysaccharide yield of(34.35±0.2)%.Antioxidant experiments revealed that a polysaccharide concentration of 5 mg/mL exhibited the highest scavenging rates for DPPH,hydroxyl radicals,and ABTS+,reaching 37.7%,96.6%,and 77.8%,respectively.This study provides an effective method for the extraction of polysaccharides from Dendrobium officinale,and explores the impact of polysaccharide concentration on antioxidant capacity,offering insights for the extraction of polysaccharides from Dendrobium officinale and the development of antioxidant additives.关键词
铁皮石斛/多糖/低共熔溶剂法/提取工艺/抗氧化活性Key words
Dendrobium officinale/polysaccharide/low eutectic solvent method/extraction process/antioxidant activity分类
轻工纺织引用本文复制引用
侯天宇,杨景瑶,胡扬凡,张芷瑜,郭海云,李河,张志军..低共熔溶剂提取晋产吸水石栽培铁皮石斛多糖工艺优化及抗氧化活性研究[J].中国食品添加剂,2024,35(6):8-15,8.基金项目
项目基金:山西省基础研究计划青年项目(202203021222032) （202203021222032）
山西省重点研发计划项目特优农业技术创新专项(2022ZDYF123) （2022ZDYF123）
