低共熔溶剂提取晋产吸水石栽培铁皮石斛多糖工艺优化及抗氧化活性研究OACSTPCD

Dendrobium officinale is a traditional plant in China used for both medicinal and edible purposes,with high traditional Chinese medicinal value,especially due to its significant polysaccharide content.Dendrobium officinale cultivated on water-absorbing stones in Shanxi province was taken as the raw materials,the polysaccharide extraction process was optimized using eutectic solvent extraction technique through single-factor and response surface analysis.Subsequently,the scavenging effects of different concentrations of polysaccharides on DPPH,hydroxyl radicals,and ABTS+were analyzed.The results showed that the optimized polysaccharide extraction process of Dendrobium officinale using a L-proline-oxalic acid eutectic solvent system was as follows:with a material-to-liquid ratio of 1∶21(g/mL),the temperature of 56℃,under ultrasound for 30 minutes,resulted in a polysaccharide yield of(34.35±0.2)%.Antioxidant experiments revealed that a polysaccharide concentration of 5 mg/mL exhibited the highest scavenging rates for DPPH,hydroxyl radicals,and ABTS+,reaching 37.7%,96.6%,and 77.8%,respectively.This study provides an effective method for the extraction of polysaccharides from Dendrobium officinale,and explores the impact of polysaccharide concentration on antioxidant capacity,offering insights for the extraction of polysaccharides from Dendrobium officinale and the development of antioxidant additives.