当归牡丹皮提取物的抗氧化活性及功效评价研究OACSTPCD

Taking the compatibility of two Chinese herbs,Angelica sinensis and Peony bark,as the research object,the contents changes of ferulic acid and paeonol were determined by HPLC with different extraction and purification processes,and the optimal extraction conditions were obtained using response surface design software.The antioxidant activity of the extracts of Angelica sinensis and Peony bark was evaluated through methods like tyrosinase inhibition assays,antimicrobial assays,and the use of the German CK multifunctional skin tester to assess their ex vivo efficacy.The results showed that when the material-liquid ratio was 1∶20,the extraction temperature was 60℃,and using 70%ethanolic ultrasonic extraction for 60 min,a substantial material content foundation was yielded.Ex vivo experiments showed that the extract of Angelica sinensis and Peony bark had strong antioxidant activity,tyrosinase activity inhibition and antibacterial effect.In vivo experiments showed that the extract of Angelica sinensis and Peony bark had the best whitening effect at the dosage ratio of 2∶1.This indicated that Angelica sinensis and Peony bark composition had potential as a natural ingredient with anti-aging,antibacterial and skin care properties.