北沙参提取物改善阴虚便秘作用研究OACSTPCD

In order to explore the effects and mechanisms of Glehniae radix aqueous extract in improving constipation and to explore the differences in different processing methods.The main components of the Glehniae radix aqueous were determined by UHPLC-HRMS method;After establishing a Yin deficiency constipation rat model with loperamide and levothyroxine sodium,mice were orally administered with two processed forms of Glehniae radix aqueous extract(1 g/kg of crude herbs)for 7 days.Metabolic indicators of rats were measured,and changes in colonic tissue morphology were observed;Alcian blue staining and immunohistochemical method was used to observe mucus secretion and detect the protein expression of 5-HT4R of mice colon tissues.The results showed that the unpeeled aqueous extract was richer in constituents than the peeled aqueous extract.After treatment with the aqueous extract of Glehniaer radix,the time of the first black stool and the rate of small intestinal propulsion were significantly improved(P＜0.05),mucin and the content of 5-HT4R in the colonic tissues was significantly increased(P＜0.05).The study elucidated that the Glehniae radix aqueous extract relieved Yin-deficiency and constipation by increasing colonic mucus secretion and repairing intestinal mucus barrier,and regulating the 5-HT pathway to stimulate intestinal smooth muscle.