中国食品添加剂2024，Vol.35Issue(6)：145-153,9.DOI:10.19804/j.issn1006-2513.2024.6.019
北沙参提取物改善阴虚便秘作用研究
Effects of Glehniae radix aqueous extract on improvement of Yin-deficiency and constipation
摘要
Abstract
In order to explore the effects and mechanisms of Glehniae radix aqueous extract in improving constipation and to explore the differences in different processing methods.The main components of the Glehniae radix aqueous were determined by UHPLC-HRMS method;After establishing a Yin deficiency constipation rat model with loperamide and levothyroxine sodium,mice were orally administered with two processed forms of Glehniae radix aqueous extract(1 g/kg of crude herbs)for 7 days.Metabolic indicators of rats were measured,and changes in colonic tissue morphology were observed;Alcian blue staining and immunohistochemical method was used to observe mucus secretion and detect the protein expression of 5-HT4R of mice colon tissues.The results showed that the unpeeled aqueous extract was richer in constituents than the peeled aqueous extract.After treatment with the aqueous extract of Glehniaer radix,the time of the first black stool and the rate of small intestinal propulsion were significantly improved(P＜0.05),mucin and the content of 5-HT4R in the colonic tissues was significantly increased(P＜0.05).The study elucidated that the Glehniae radix aqueous extract relieved Yin-deficiency and constipation by increasing colonic mucus secretion and repairing intestinal mucus barrier,and regulating the 5-HT pathway to stimulate intestinal smooth muscle.关键词
北沙参/阴虚便秘/结肠/5-HT4RKey words
Glehniae radix/Yin-deficiency and constipation/colon/5-HT4R分类
轻工业引用本文复制引用
杨宇鑫,李凯鹏,王常顺,贾东升,王嫚,赵丁..北沙参提取物改善阴虚便秘作用研究[J].中国食品添加剂,2024,35(6):145-153,9.基金项目
河北省自然科学基金项目(C2022206018,H2023206068) （C2022206018,H2023206068）
河北省重点研发计划项目(21372502D) （21372502D）
