5种中草药提取液对糙皮侧耳发酵产麦角硫因的影响OACSTPCD

In order to enhance the production of ergothioneine through liquid fermentation of edible fungi,the effects of five Chinese herbal extracts(Codonopsis,Poria,Coix Kernel,Goji Berry and Licorice)on yield of ergothioneine were investigated using Pleurotus ostreatus P105 as the fermentation strain in shake flask fermentation.Analysis of mycelium biomass and ergothioneine yield revealed that the addition of the Chinese herbal compound extract significantly improved outcomes compared to the control.Notably,the combination of goji berry and codonopsis extracts was most effective,yielding a mycelial biomass of 25.89 g/L(7.31 g/L higher than the control)and an ergothioneine yield of 7.67 mg/L(1.85 mg/L higher than the control).The response surface analysis method was used to optimize the fermentation process.The optimal fermentation conditions were as follows:the ratio of goji berry/codonopsis extract of 2.1∶1,pH 6.5,agitation speed of 165 r/min,and inoculation amount of 8％.Under these conditions,the yield of ergothioneine could reach 8.53 mg/L,which was 4.61 times higher than the pre-optimization yield.This research suggests a novel approach and provides a theoretical foundation for using Chinese herbal extracts to enhance ergothioneine production in Pleurotus ostreatus fermentation.