药桑、两色金鸡菊、刺山柑提取物对α-葡萄糖苷酶的抑制作用研究OACSTPCD

Taking three distinctive Xinjiang dual-purpose food and medicinal resources,Morus nigra L.,Coreopsis tinctoria Nutt.,and Capparis spinosa L.,as research subjects,with the commonly used clinical drug acarbose as the positive control,classical a-glucosidase activity determination methods were employed to screen the enzyme inhibition activity of their water and alcohol extracts.The extract with the strongest inhibitory effect was subjected to enzyme kinetic analysis.Morus nigra L.,Coreopsis tinctoria Nutt.,and Capparis spinosa L.extracts exhibited significant inhibitory effects on α-glucosidase.The IC50 values were as follows:Morus nigra L.alcohol extract 2.636 μg·mL-1,Morus nigra L.water extract 7.948 μg·mL-1,Coreopsis tinctoria Nutt.alcohol extract 0.000645μg·mL-1,Coreopsis tinctoria Nutt.water extract 6.962 pg·mL-1,Capparis spinosa L.alcohol extract 78.11 μg·mL-1,Capparis spinosa L.water extract 88.5 μg·mL-1,and acarbose 24.68 μg·mL-1.Enzyme kinetics experiments revealed that Coreopsis tinctoria Nutt.alcohol extract exhibited reversible competitive inhibition against α-glucosidase.Both the water and alcoholic extracts of Coreopsis tinctoria Nutt.and Morus nigra L.exhibited promising a-glucosidase inhibitory activities,indicating their potential as food and medicine dual-use products for managing postprandial blood glucose levels,with high value for the further research.