槐米的红外快速鉴别与总黄酮、总酚酸、还原糖含量测定OACSTPCD

To study the total component content of Sophora japonica flower buds,Fourier infrared spectroscopy was used to qualitatively study Sophora japonica flower bud samples.Aluminum nitrate chromogenic method,forinphenol method and DNS were used to determine total flavonoids,total phenolic acid and reducing sugars of Sophora japonica flower buds.The peak shapes of the infrared spectra of 14 batches of Sophora japonica flower buds were consistent,and the one-dimensional infrared spectra of each batch exhibited characteristic absorption peaks characterizing phenolic acids,flavonoids and carbohydrates.In its second-derivative infrared spectra,the presence of flavonoids was further confirmed.The content determination revealed that it was rich in total flavonoids,total polyphenols,and reducing sugars.Sophora japonica flower buds was rich in phenolic acids,flavonoids and reducing sugar components,and the combination of one-dimensional infrared spectroscopy and second-derivative spectroscopy could provide scientific data for the identification and analysis of different batches of Sophora japonica flower buds,aiding in overall quality control of Sophora japonica flower buds and the qualitative analysis of active ingredients;this study provides theoretical support for the later development and utilization of Sophora japonica flower buds,and a reference for controlling the quality of medicinal materials of Sophora japonica flower buds and ensuring its safe use.