食品与机械2024，Vol.40Issue(7)：1-6,80,7.DOI:10.13652/j.spjx.1003.5788.2023.81195
人参皂苷Rb1脂质体的制备及对脂肪细胞中脂滴积聚的抑制作用
Investigating the inhibitory effect of β-Rb1-Lip on lipid droplet accumulation in 3T3-L1 adipocytes
摘要
Abstract
[Objective]β-Sitosterol-modified ginsenoside Rb1 liposomes(β-Rb1-Lip)were prepared to reduce the degradation of Rb1 and enhance the lipid-lowering effects of ginsenoside Rb1.[Methods]β-sitosterol-modified ginsenoside Rb1 liposomes were prepared by the thin-film hydration method.The biosafety of the liposomes was assessed using the MTT assay.The inhibition of lipid droplet accumulation in 3T3-L1 adipocytes by β-Rb1-Lip was investigated using Oil Red O staining and triglyceride(TG)content measurement with an enzyme-linked immunosorbent assay.[Results]The prepared β-Rb1-Lip liposomes had an encapsulation efficiency of 83.74％and an average particle size of 198 nm.The release rate of Rb1 from β-Rb1-Lip was about 80％within 12 hours,demonstrating a good sustained-release effect.Regarding lipid-lowering activity,β-Rb1-Lip at 50 μmol/L showed a significant inhibitory rate of intracellular lipid droplets.Compared to the same concentration of Rb1 monomer,β-Rb1-Lip had a more significant inhibitory effect on the accumulation of lipid droplets in 3T3-L1 cells and did not exhibit cytotoxicity.[Conclusion]β-Rb1-Lip has high encapsulation efficiency,small particle size,and obvious sustained-release characteristics.It can continuously release the active component ginsenoside Rb1,enhance its lipid-lowering efficacy,and reduce the dosage,providing support for the development of Rb1 lipid-lowering products.关键词
人参皂苷Rb1/β-谷甾醇/3T3-L1前脂肪细胞/脂滴积聚/脂质体Key words
ginsenoside Rb1/β-sitosterol/3T3-L1 preadipocytes/lipid droplet accumulation/liposome引用本文复制引用
尤晓颜,刘慧,段续,陈玥..人参皂苷Rb1脂质体的制备及对脂肪细胞中脂滴积聚的抑制作用[J].食品与机械,2024,40(7):1-6,80,7.基金项目
国家自然科学基金项目(编号:31200035) （编号:31200035）
