人参皂苷Rb1脂质体的制备及对脂肪细胞中脂滴积聚的抑制作用OA北大核心CSTPCD

[Objective]β-Sitosterol-modified ginsenoside Rb1 liposomes(β-Rb1-Lip)were prepared to reduce the degradation of Rb1 and enhance the lipid-lowering effects of ginsenoside Rb1.[Methods]β-sitosterol-modified ginsenoside Rb1 liposomes were prepared by the thin-film hydration method.The biosafety of the liposomes was assessed using the MTT assay.The inhibition of lipid droplet accumulation in 3T3-L1 adipocytes by β-Rb1-Lip was investigated using Oil Red O staining and triglyceride(TG)content measurement with an enzyme-linked immunosorbent assay.[Results]The prepared β-Rb1-Lip liposomes had an encapsulation efficiency of 83.74％and an average particle size of 198 nm.The release rate of Rb1 from β-Rb1-Lip was about 80％within 12 hours,demonstrating a good sustained-release effect.Regarding lipid-lowering activity,β-Rb1-Lip at 50 μmol/L showed a significant inhibitory rate of intracellular lipid droplets.Compared to the same concentration of Rb1 monomer,β-Rb1-Lip had a more significant inhibitory effect on the accumulation of lipid droplets in 3T3-L1 cells and did not exhibit cytotoxicity.[Conclusion]β-Rb1-Lip has high encapsulation efficiency,small particle size,and obvious sustained-release characteristics.It can continuously release the active component ginsenoside Rb1,enhance its lipid-lowering efficacy,and reduce the dosage,providing support for the development of Rb1 lipid-lowering products.