再生丝素蛋白在Na+溶液中的分子聚集行为及生物学性能研究OA北大核心CSTPCD

Objective:The aggregation behavior of silk fibroin under specific conditions can influence its kinetic behavior and viscoelastic properties,thereby affecting biological properties of silk fibroin materials.Therefore,this study systematically investigated the self-assembly aggregation behavior of silk fibroin and explored the conditions for enhancing its mechanical performance and biological properties.Methods:Regenerated silk fibroin was extracted and prepared from domestic silkworm cocoons,and the optimal assembly conditions were determined through turbidity experiments.Rheological experiments were conducted to study the influence of Na+on the kinetic behavior and viscoelastic properties of silk fibroin solutions.Cell proliferation experiments were carried out to investigate the biological properties of silk fibroin.Results:The extracted regenerated silk fibroin primarily exhibited a random coiled structure.The optimal in vitro self-assembly conditions involved assembly of a 4 mg/mL solution at 70 ℃ for 6 hours.The addition of Ca2+to the silk fibroin solution inhibited in vitro self-assembly,while the addition of Na+accelerated the assembly rate and increased the degree of assembly.Furthermore,with increasing Na+concentration,the gel strength of silk fibroin gradually increased,leading to a more stable gel network.Cell proliferation experiments demonstrated that the solution of regenerated silk fibroin was more conducive to cell growth and proliferation than its self-assembled fibers.Conclusion:The concentration,temperature,assembly time,and ions all have an impact on the in vitro self-assembly aggregation behavior of silk fibroin.This study provides preliminary research on the self-assembly aggregation conditions and biological properties of silk fibroin,offering experimental basis for improving the performance of silk fibroin as a food packaging material.