食品与机械2024，Vol.40Issue(8)：16-22,39,8.DOI:10.13652/j.spjx.1003.5788.2024.80232
差异化蛋白脱除对早籼米粉性质的影响
Effects of differential protein removal on the properties of early indica rice flour
摘要
Abstract
[Objective]To investigate the mechanism of the effect of deproteinisation on the physicochemical and functional properties of rice flour.[Methods]Enzymatic and alkaline methods were used to remove protein from early rice flour,and the damaged starch content,size distribution,morphology,solubility,swelling power,pasting characteristics and textural characteristics of the deproteinized rice flour were analyzed.[Results]Sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE)and confocal laser scanning microscope(CLSM)showed that the alkaline method equally removed the protein subunit where as the enzymatic method showed a better homogeneity of protein removal from the surface of starch.The size distribution of the control showed a majorpeak at ca.70 μm and it shifted to 6μmafter protein removal.With the reduction of the protein content,and the damaged starch content of the deproteinized rice flour decreased while its elasticity and hardness increased.The deproteinized rice flour with 3.0%of protein(RFA@3.0),which was treated by alkaline method,showed a most uniform particle distribution,and a minimum D90 of 23.58 μm was observed.When compared to the control,its damaged starch content decreased from 3.32%to 1.48%while the elasticity and hardness increased by 54.55%,52.60%,respectively.The deproteinized rice flour had lower peak viscosity,breakdown viscosity and final viscosity than the control,and the RFA@3.0 had minimum breakdown and setback viscosities,which indicated that it exhibited best paste stability and was difficult to retrograde.[Conclusion]The protein removal by alkaline and enzymatic methods can improve the properties of early indica rice flour.The enzymatic method has mild reaction conditions and the result and products has better protein and size distribution and are more difficult to retrograde than the rice flour that treated by alkaline method.关键词
早籼米粉/脱蛋白/碱法/酶法Key words
early indica rice flour/deproteinization/alkaline method/enzymatic method引用本文复制引用
曹怡君,冯伟,王韧,张昊,王涛..差异化蛋白脱除对早籼米粉性质的影响[J].食品与机械,2024,40(8):16-22,39,8.基金项目
国家重点研发计划项目(编号:2023YFD2100803) （编号:2023YFD2100803）
