大孔吸附树脂纯化芦笋皂苷工艺优化OA北大核心CSCDCSTPCD
Purification of saponins from Asparagus officinalis by macroporous adsorption resin
以芦笋下脚料提取物作为原料,优化了大孔吸附树脂纯化芦笋皂苷的工艺。通过静态法,对8种大孔吸附树脂纯化芦笋皂苷的效果进行比较,结果表明HPD-100树脂纯化芦笋皂苷效果最佳,并对HPD-100大孔树脂纯化芦笋皂苷工艺进行了优化。在上样质量浓度15mg/mL、溶液处理量150mL、1mL/min流速过柱;吸附后,先用50mL蒸馏水洗去杂质,再用200mL80%乙醇洗脱,总皂苷的含量由14.26%提高到35.49%（mean±SD）。将初次纯化产…查看全部>>
An optimal macroporous adsorption resin and its optimal technological parameters for purification of saponins from extracts of Asparagus officinalis were investigated in the present study.Through static method,comparing the saponin purification result of 8 resins,HPD-100 resin was chosen due to its excellent adsorption and desorption performance.Optimal conditions were primarily confirmed as follows：sample concentration 15mg/mL with loading amount of 1…查看全部>>
赵芳春;赵兵;黄云祥
中国科学院过程工程研究所生化工程国家重点实验室,北京100190 中国科学院研究生院,北京100049中国科学院过程工程研究所生化工程国家重点实验室,北京100190秦皇岛长胜农业科技发展有限公司,河北秦皇岛066000
轻工纺织
芦笋皂苷大孔吸附树脂纯化HPD-100
Asparagus officinalissaponinsmacroporous adsorption resinpurificationHPD-100
《食品工业科技》 2012 (18)
248-252,5
科技部农业科技成果转化资金项目（2010A20007）
评论