不同方法提取红鳍笛鲷鱼鳞胶原蛋白的结构和理化特性研究OA北大核心CSTPCD

To explore the impact of various extraction techniques on the physicochemical properties of collagen in Lutjanus erythropter-us scales,acid-soluble collagen(ASC),pepsin-soluble collagen(PSC),and hot water-soluble collagen(HSC)were extracted.The ex-traction rates,yields,and purities of the three methods were compared and analyzed.The amino acid composition,molecular weight,sec-ondary structure,Zeta potential,solubility,and microstructure of both collagens were evaluated.The findings revealed that the highest col-lagen extraction rate was achieved using the hot water method(45.25％),followed by the pepsin method.The imide acid content of HSC,ASC,and PSC was 18.28％,17.54％,and 18.30％,respectively.All collagens were identified as type Ⅰ collagen,consisting of two a chains(αl and α2).ASC exhibited a higher β chain content than PSC,while both HSC and PSC lacked the γ band.ASC and PSC dis-played similar IR spectra and maintained the intact triple helix structure,whereas hot water extraction resulted in triple helix degradation.Zeta potential analysis revealed a net charge of zero for HSC,ASC,and PSC within the pH range of 5-6.ASC and PSC exhibited high solubility within a neutral to alkaline pH range(5-11).Scanning electron microscope results illustrated that ASC possessed a disordered fibrous structure,PSC had a dense porous network structure,and HSC appeared primarily laminar.Although all three extraction methods yielded type 1 collagen,their structural and physicochemical attributes differed.Therefore,specific collagen extraction methods can be se-lected to develop diverse products based on specific requirements.