大蝎子草总多酚提取工艺优化及抗氧化活性OA北大核心CSTPCD

The ultrasonic-assisted ethanol was used to extract the total polyphenols from the aerial part of Girardinia diversifolia in this experiment.Box-Behnken model was designed based on the results of single-factor experiments to optimize the optimal process conditions of total polyphenols from the aerial part of Girardinia diversifolia.DPPH free radical scavenging rate and FRAP method were used to evalu-ate the antioxidant capacity of total polyphenols from the aerial part of Girardinia diversifolia.Results showed when the optimal process parameters as ethanol concentration,liquid-solid ratio,ultrasonic time and ultrasonic temperature were 75％,50 mL/g,35 min and 40 ℃,respectively,the extraction rate of to-tal polyphenols was 2.81％,which was consistent with the predicted value of Box-Behnken model.The an-tioxidant test showed that the DPPH radical-scavenging activity of 0.15 mg/mL total polyphenol from the aerial part of Girardinia diversifolia could reach 75.8％,and the IC50 value of the half inhibitory concen-tration was 0.057 mg/mL,which had certain antioxidant capacity.It provides corresponding theoretical da-ta support for further research and utilization of wild resources of Girardinia diversifolia,and a theoreti-cal basis for the development of new feed additives.