家畜生态学报2024，Vol.45Issue(6)：50-56,7.DOI:10.3969/j.issn.1673-1182.2024.06.008
大蝎子草总多酚提取工艺优化及抗氧化活性
Optimization of Extraction Process and Antioxidant Activity of Total Polyphenols from Girardinia diversifolia
摘要
Abstract
The ultrasonic-assisted ethanol was used to extract the total polyphenols from the aerial part of Girardinia diversifolia in this experiment.Box-Behnken model was designed based on the results of single-factor experiments to optimize the optimal process conditions of total polyphenols from the aerial part of Girardinia diversifolia.DPPH free radical scavenging rate and FRAP method were used to evalu-ate the antioxidant capacity of total polyphenols from the aerial part of Girardinia diversifolia.Results showed when the optimal process parameters as ethanol concentration,liquid-solid ratio,ultrasonic time and ultrasonic temperature were 75％,50 mL/g,35 min and 40 ℃,respectively,the extraction rate of to-tal polyphenols was 2.81％,which was consistent with the predicted value of Box-Behnken model.The an-tioxidant test showed that the DPPH radical-scavenging activity of 0.15 mg/mL total polyphenol from the aerial part of Girardinia diversifolia could reach 75.8％,and the IC50 value of the half inhibitory concen-tration was 0.057 mg/mL,which had certain antioxidant capacity.It provides corresponding theoretical da-ta support for further research and utilization of wild resources of Girardinia diversifolia,and a theoreti-cal basis for the development of new feed additives.关键词
总多酚/大蝎子草/超声提取/抗氧化Key words
total polyphenols/Girardinia diversifolia/ultrasonic extraction/antioxidant分类
农业科技引用本文复制引用
高林晓,石景海,勾娜,伍泽波,管永庆,郭蒙..大蝎子草总多酚提取工艺优化及抗氧化活性[J].家畜生态学报,2024,45(6):50-56,7.基金项目
贵州省教育厅青年科技人才成长项目(黔教合KY字[2018]440,[2015]389) （黔教合KY字[2018]440,[2015]389）
国家级大学生创新创业训练项目(202110670005) （202110670005）
黔南民族师范学院校级科研项目(2020qnsy14) （2020qnsy14）
贵州省普通高校民族药用植物资源开发工程研究中心开放基金(黔教合KY字[2014]227) （黔教合KY字[2014]227）
